Arichard:Samples/sort150408
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- 2 tubes sorted into PBSE + 1% BSA (sat in tube at least 1 hour prior)
- Full PI NeuN+ CTX from AD patient 18-99 (100,000)
- Full PI NeuN- CTX from AD patient 18-99 (15,000)
- Spun down at 900 RCF for 10 min at 4 C in bucket rotor
- Resuspended in PBS + 1% BSA (100 ul for NeuN+, 30 ul for NeuN-), filtered through CellTrics 20 um filter, counted on BioRad TC20
- NeuN+ was 350/ul
- NeuN- was 450/ul
- Aliquoted NeuN+ in 5 ul volume, 15 tubes (Labeled +)
- Aliquoted NeuN- in 2 ul volume, 10 tubes (Labeled -)