Athurva Gore/LabNotes/2011-10-5

From ZhangLabWiki
Jump to navigation Jump to search

TO DO[edit]

  • Free space on Genome-Miner, create Shared Folder for HiSeq - DONE
    • Monitor run; if necessary, free up more disk space
  • Nature Methods Manuscript
    • Barcoding Padlock Probe libraries - Need to meet with Dinh and Noi
  • Goldstein Lab Samples
    • Did not end up having time to do PCR today; run reactions tomorrow morning
    • Can get results by Friday instead of tomorrow
  • Cell Culture
    • Tonight, look at schedule for CERC tomorrow
    • Need to finish EB work tomorrow; email Christine or Carl about this
  • Low-input Bisulfite Conversion
  • Paper with Sergio (see notes from August 22nd)
    • Obtained mRNA sequences for other HUV and AST iPS mutated genes
    • Need to design primers that work on cDNA for these samples
  • Low-input Exomes (Athanasia)
  • Papers for Doug - ask Dr. Zhang?

Nature Methods Manuscript[edit]

Meeting with Dinh and Noi[edit]

  • See google document here: [[1]]
  • Came up with list of Action items
  • My responsibilities:
    • Disclose Probe Design Algorithm - Athurva
      • Write paragraph on algorithm
      • Mention adaptability of algorithm to different organisms and target regions
      • Generate figure describing algorithm
      • Package software for distribution
      • Include a manual on how to use the software
    • Edit Main Text
    • Help with barcoding experiments