Blue:RNA-Seq Experiments:03122013
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Purpose: Improve RNA fragmentation in RNAseIII single cell totoRNAseq protocol
Results: Pre-treated cells with T20 primer to protect polyA tail - incubated at 72C for 3 minutes prior to adding RNAseIII - treated with RNAse for 20min rather than 10min. Similar low level of PCR amplification observed but fragmentation sizes were in the expected range (200-800bp). Libraries lost following DSN treatment