Blue:RNA-Seq Experiments:03212013
Jump to navigation
Jump to search
Purpose: Test RNAseIII single cell totoRNAseq protocol - Same as for 03192013 except with a longer RNAseIII incubation time (increased from 10min to 20min)
Result: Only minor downward shift in library fragment size - still too many high molecular weight fragments and poor amplification after DSN treatment