Blue:RNA-Seq Experiments:03252013
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Purpose: (1) Test single cell totoRNAseq protocols on genomic DNA compared to UHRR - Determine if gDNA contributes to final library after RT-based fragmentation or RNaseIII based fragmentation protocols. (2) Test commercial RNaseIII buffer with homemade RNaseIII buffer.
Results: (1) No gDNA contribution to RNA-seq library even after extended high temperature treatments that are used for cell lysis in these protocols. (2) Homemade RNaseIII buffer is comparable with commercial buffer