Blue:RNA-Seq Experiments:03282013
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Purpose: Test effects of Triton-X100 and RNase Inhibitor (components of cell lysis buffer) on the single cell totoRNAseq protocol using RNaseIII RNA fragmentation of UHRR
Results: (1) 0.2% Triton X-100 slighly reduced library amplification (2) RNase Inhibitor slighly elevated fragmentation spread. Therefore, both components might reduce efficiency in the single cell context (which involves 1:10 dilution of RNaseIII or MMLV).