Blue:RNA-Seq Experiments:04162013
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Purpose: Test the effects of lysis buffer on RT fragmentation-based single cell RNA-seq protocol
Results: Tested RT buffer with or without Triton-x100 (0.1%, 0.2%), NP-40 (0.1%, 0.2%, 0.5%) or RNase Inhibitor. Used 72C 10 min followed by 94C 5 min. A lot of evaporation occurred and the RNA looked over-fragmented.