Blue:RNA-Seq Experiments:04302014

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Whole Sort and Tissue Control Libraries[edit]

RNA Extractions

  • Samples:
    • 20140213 (BA8) - Tissue Chunk
    • 20140213 (BA8) - Bulk Sort 100K
    • 20140410 (BA17) - Tissue Chunk
    • 20140410 (BA17) - Bulk Sort 100K
    • 20140415 (BA21) - Tissue Chunk
    • 20140415 (BA21) - Bulk Sort 80K
    • 20140417 (BA10) - Tissue Chunk
    • 20140417 (BA10) - Bulk Sort 100K
    • 20140422 (BA22) - Tissue Chunk
    • 20140422 (BA22) - Bulk Sort 80K
    • 20140424 (BA41/42) - Tissue Chunk
    • 20140424 (BA41/42) - Bulk Sort 50K
  • RNA extracted with Zymo ZR RNA Microprep Kit (including DNaseI treatment)
  • RNA concentrations determined using Qubit:
Sample Sample Concentration (ng/ul) Est. RNA/Nuclei (pg)
20140213 (BA8) - Tissue Chunk 29.3
20140213 (BA8) - Bulk Sort 100K 7.54 0.754
20140410 (BA17) - Tissue Chunk 55.3
20140410 (BA17) - Bulk Sort 100K 27.4 2.74
20140415 (BA21) - Tissue Chunk 43.5
20140415 (BA21) - Bulk Sort 80K 18.8 2.35
20140417 (BA10) - Tissue Chunk 53.5
20140417 (BA10) - Bulk Sort 100K 14.9 1.49
20140422 (BA22) - Tissue Chunk 63.9
20140422 (BA22) - Bulk Sort 80K 20.6 2.575
20140424 (BA41/42) - Tissue Chunk 57.3
20140424 (BA41/42) - Bulk Sort 50K 41.4 8.28