Blue:RNA-Seq Experiments:05172013
Jump to navigation
Jump to search
Library: UHRR from 05162013
Purpose: Test for optimal annealing temperature for PB_PCR_F/PGR_PCR_R using Sybr
Outcome: Optimal range between 58C and 65C, drop in efficiency above 65C. Also poor amplification if only the first stage of the PCR protocol is used.