Blue:RNA-Seq Experiments:05222013
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Library: BL_mESC_RNaseIII_N2id1-96_0522
Purpose: Test run of the RNaseIII-based single cell totoRNAseq protocol using optimized PhusionHF amplification rather than Advantage
Outcome: PhusionHF did not prevent the ssDNA library amplification problem. Therefore, PCR amplification is not the main cause of this problem. Likely the TSO step is sub-optimal.