Blue:RNA-Seq Experiments:06032013

From ZhangLabWiki
Jump to navigation Jump to search

Library: BL_mESC_RT_N2id1-96_0603

Purpose: Test run of the Mg-based single cell totoRNAseq protocol with the following modifications: (1) 1uM T20; (2) 1/5 diluted PNK; (3) 20 or 50U MMLV; (4) 10uM TSO.R04; (5) PhusionHF amplification.

Outcome: Still significant issue with single stranded library amplification, but looks like undiluted enzymes/primers works better.


File:06032013.pdf