Blue:RNA-Seq Experiments:06062013
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Purpose: Optimize PCR amplification and template switching. Tested PhusionHF versus KAPA-HF; tested concentration of TSO (1uM versus 10uM); Tested TSO.r04 versus LNA TSO.r05.
Outcome: PhusionHF works the best for amplification. 10uM TSO.r04 works significantly better than 1uM. TSO.r04 and r05 have similar efficiency.