Blue:RNA-Seq Experiments:06132013
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Purpose: Optimizations. Tested r04 versus r05 using 1ng RNA to reduce chances for variability seen with 06122013 experiment. Also tested betaine again as well as one step T20/TSO without beads purification prior to PCR amplification.
Outcome: Betaine does seem to improve efficiency. r05 may improve efficiency slightly. One-step without beads purification increases efficiency but leads to worse primer dimer effect compared to prior beads purified test.