Blue:RNA-Seq Experiments:09052013

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Fluidigm C1 Small Chip Beta Test[edit]

  • Tested NPCs on the small 5-10um chip
  • First run on 09-04-2013 produced too few viable cells for loading and resulted in only 10 captured - therefore, reprimed the chip and stored overnight for re-loading
  • Second run on 09-05-2013 involved E13.5 neural progenitors sorted as PI negative
  • Achieved good loading efficiency (81/96) but high mortality as indicated by live/dead staining on chip
  • Followed the protocol exactly - including use of spike-in RNA indicated in the protocol rather than those used in our previous experiments

OutCome:

  • Re-primed incorrectly - broke seal on chip preventing lysis buffer from entering chambers - no cDNA amplification


File:Cell Characterization Worksheet.docx

File:Data Submission Worksheet.docx

Sample Attribution[edit]

Note: d=dead


' 1 2 3 4 5 6 7 8 9 10 11 12
A 1_NPCs_C03 0_NPCs_C02 1d_NPCs_C01 0_NPCs_C49 1d_NPCs_C50 1d_NPCs_C51 1d_NPCs_C06 0_NPCs_C05 1_NPCs_C04 2d_NPCs_C52 0_NPCs_C53 3d_NPCs_C54
B 1d_NPCs_C09 0_NPCs_C08 0_NPCs_C07 1d_NPCs_C55 1d_NPCs_C56 1d_NPCs_C57 1d_NPCs_C12 1d_NPCs_C11 1d_NPCs_C10 1d_NPCs_C58 0_NPCs_C59 4d_NPCs_C60
C 1d_NPCs_C15 1d_NPCs_C14 0_NPCs_C13 1_NPCs_C61 0_NPCs_C62 1d_NPCs_C63 1_NPCs_C18 1_NPCs_C17 1_NPCs_C16 1d_NPCs_C64 1d_NPCs_C65 1d_NPCs_C66
D 1d_NPCs_C21 1d_NPCs_C20 0_NPCs_C19 1_NPCs_C67 1d_NPCs_C68 1d_NPCs_C69 2d_NPCs_C24 1d_NPCs_C23 1d_NPCs_C22 0_NPCs_C70 1d_NPCs_C71 1 + 1d_NPCs_C72
E 1d_NPCs_C25 3d_NPCs_C26 1_NPCs_C27 0_NPCs_C75 1d_NPCs_C74 0_NPCs_C73 2d_NPCs_C28 2d_NPCs_C29 1d_NPCs_C30 0_NPCs_C78 1d_NPCs_C77 2 + 1d_NPCs_C76
F 4d_NPCs_C31 1_NPCs_C32 1d_NPCs_C33 1_NPCs_C81 1d_NPCs_C80 2d_NPCs_C79 1d_NPCs_C34 1d_NPCs_C35 3 + 1d_NPCs_C36 1d_NPCs_C84 1_NPCs_C83 1_NPCs_C82
G 1d_NPCs_C37 0_NPCs_C38 1_NPCs_C39 1d_NPCs_C87 2d_NPCs_C86 1 + 1d_NPCs_C85 1_NPCs_C40 1_NPCs_C41 1d_NPCs_C42 2d_NPCs_C90 1_NPCs_C89 1_NPCs_C88
H 1d_NPCs_C43 4d_NPCs_C44 1d_NPCs_C45 1d_NPCs_C93 1d_NPCs_C92 4d_NPCs_C91 3d_NPCs_C46 1d_NPCs_C47 1d_NPCs_C48 1d_NPCs_C96 1d_NPCs_C95 1d_NPCs_C94