Blue:RNA-Seq Experiments:10062014

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Size Analysis of Mouse DRG Nuclei[edit]

  • Recieved DRG nuclei (Mouse P2) from Ganlu (Guoping Fan Lab, UCLA) on 10-03-2014
  • Diluted nuclei to 600/ul, ran on biorad - one tight peak at 5um
  • Checked under microscope - extensive clusters
  • Filtered with 20um filter to clean up - removed many clusters - dropped [] to 250/ul
  • Measured nuclei width using microscope (10x objective) and Metamorph software:
Nuclei Width (um)
6.45
9.03
6.45
5.8
9.03
5.8
4.52
7.21
5.95
5.32
7.1
7.1
6.45
5.8
7.1
7.74
5.8
5.8
5.8
5.8
7.1
5.16
5.8
8.48
10.32
7.1
5.95
5.32
5.8
7.1
9.68
6.45
Mean = 6.6971875