Blue:RNA-Seq Experiments:11052014

From ZhangLabWiki
Jump to navigation Jump to search

Miseq QC Libraries[edit]

huNu C1 Libraries:

  • 20141015A
  • 20141015B
  • 20141022A
  • 20141022B


Library Preparation

  • Noramalized cDNA to 0.2ng/ul
  • Prepared Nextera as per C1 instructions
  • Beads purified libraries twice using 0.8x beads
  • Reconstituted each library in 10ul and ran 1ul on gel:


File:10152014 PCR2 Gel1.jpg

Libraries Sample Barcode
BL_20141015A_1C03_Nov5 20141015A_1C03 S501/N701
BL_20141015A_1C14_Nov5 20141015A_1C14 S501/N702
BL_20141015A_1C49_Nov5 20141015A_1C49 S501/N703
BL_20141015A_1C86_Nov5 20141015A_1C86 S501/N704
BL_20141015A_0C15_Nov5 20141015A_0C15 S502/N701
BL_20141015A_1C26_Nov5 20141015A_1C26 S502/N702
BL_20141015A_1C55_Nov5 20141015A_1C55 S502/N703
BL_20141015A_1C92_Nov5 20141015A_1C92 S502/N704
BL_20141015B_1C32_Nov5 20141015B_1C32 S505/N710
BL_20141015B_1C61_Nov5 20141015B_1C61 S505/N711
BL_20141015B_0C25_Nov5 20141015B_0C25 S506/N709
BL_20141015B_1C38_Nov5 20141015B_1C38 S506/N710
BL_20141015B_1C75_Nov5 20141015B_1C75 S506/N711
BL_20141015B_1C31_Nov5 20141015B_1C31 S507/N709
BL_20141015B_1C44_Nov5 20141015B_1C44 S507/N710
BL_20141015B_1C87_Nov5 20141015B_1C87 S507/N711
BL_20141022A_0C49_Nov5 20141022A_0C49 S503/N705
BL_20141022A_1C92_Nov5 20141022A_1C92 S503/N706
BL_20141022A_1C18_Nov5 20141022A_1C18 S503/N707
BL_20141022A_1C35_Nov5 20141022A_1C35 S503/N708
BL_20141022A_1C67_Nov5 20141022A_1C67 S504/N705
BL_20141022A_1C69_Nov5 20141022A_1C69 S504/N706
BL_20141022A_1C28_Nov5 20141022A_1C28 S504/N707
BL_20141022A_1C41_Nov5 20141022A_1C41 S504/N708
BL_20141022B_1C08_Nov5 20141022B_1C08 S501/N712
BL_20141022B_1C14_Nov5 20141022B_1C14 S502/N712
BL_20141022B_1C20_Nov5 20141022B_1C20 S503/N712
BL_20141022B_1C26_Nov5 20141022B_1C26 S504/N712
BL_20141022B_1C38_Nov5 20141022B_1C38 S505/N712
BL_20141022B_0C01_Nov5 20141022B_0C01 S506/N712
BL_20141022B_1C07_Nov5 20141022B_1C07 S507/N712
BL_20141022B_1C19_Nov5 20141022B_1C19 S508/N712