Blue:RNA-Seq Libraries:05282013
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Library: BL_UHRR_IVT_0528
Purpose: Test the IVT RNA-seq protocol on 1ng and 10ng of UHRR using either a one-step or a two-step polyA T7 promoter adaptor addition. Also tested random cDNA synthesis using N6 and N9 primers. Results: Only two-step protocol worked. Both N6 and N9 primers appeared to work for fragment amplification between 200-600bp.
Note: Used T20V.id for barcoding two-step protocol.
T20V.ID1 = 10ng N6 Library
T20V.ID2 = 1ng N6 Library
T20V.ID4 = 10ng N9 Library
T20V.ID5 = 1ng N9 Library