Blue:RNA-Seq Libraries:2014

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Library Condition RNA Spike-in T20-30 (Barcode) TSO PCR Primers Library Processing N2/Nextera Index Run Type, Minmum BP Read 1 Sequencing Primer Index Primer Read 2 Sequencing Primer Seq Lane File File Location
BL_totoRNAseq_1ng-100pg-Mg-UHRR_Mar05 1ng UHRR; 2min Mg Frag; Enz. PAP 5min 1:2E5 ERCC T20VN_id1 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 1 SE 50+7 STRT-SEQ v3 N2IndSeq N/A Lane 5 s_5_1_Indx01.txt 140514_HL166
1ng UHRR; 2min Mg Frag; Enz. PAP 5min 1:2E5 ERCC T20VN_id1 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 2 SE 50+7 STRT-SEQ v3 N2IndSeq N/A Lane 5 s_5_1_Indx02.txt 140514_HL166
100pg UHRR; 2min Mg Frag; Enz. PAP 5min 1:2E6 ERCC T20VN_id1 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 3 SE 50+7 STRT-SEQ v3 N2IndSeq N/A Lane 5 s_5_1_Indx03.txt 140514_HL166
100pg UHRR; 2min Mg Frag; Enz. PAP 5min 1:2E6 ERCC T20VN_id1 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 4 SE 50+7 STRT-SEQ v3 N2IndSeq N/A Lane 5 s_5_1_Indx04.txt 140514_HL166
NTC; 2min Mg Frag; Enz. PAP 5min 1:2E7 ERCC T20VN_id11 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 6 SE 50+7 STRT-SEQ v3 N2IndSeq N/A Lane 5 s_5_1_Indx06.txt 140514_HL166
BL_totoRNAseq_1ng-100pg-RIII-UHRR_Mar05 1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E5 ERCC T20VN_id1 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 7 SE 50+7 STRT-SEQ v3 N2IndSeq N/A Lane 5 s_5_1_Indx07.txt 140514_HL166
1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E5 ERCC T20VN_id1 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 8 SE 50+7 STRT-SEQ v3 N2IndSeq N/A Lane 5 s_5_1_Indx08.txt 140514_HL166
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E6 ERCC T20VN_id1 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 9 SE 50+7 STRT-SEQ v3 N2IndSeq N/A Lane 5 s_5_1_Indx09.txt 140514_HL166
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E6 ERCC T20VN_id1 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 10 SE 50+7 STRT-SEQ v3 N2IndSeq N/A Lane 5 s_5_1_Indx10.txt 140514_HL166
NTC; 1min 0.1U RIII Frag; Affy. PAP 4min 1:2E7 ERCC T20VN_id11 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 12 SE 50+7 STRT-SEQ v3 N2IndSeq N/A Lane 5 s_5_1_Indx12.txt 140514_HL166
BL_totoRNAseq_10x10pg-Mg-UHRR_Mar05 10x10pg UHRR; 2min Mg Frag; Enz. PAP 5min 1:2E7 ERCC T20VN_id1-10 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 5 PE 50+7+8 STRT-SEQ v4 N2IndSeq N2RevSeq2 Lane 5 s_5_1_Indx05.txt 140514_HL166
BL_totoRNAseq_10x10pg-RIII-UHRR_Mar05 10x10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min 1:2E7 ERCC T20VN_id1-10 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 11 PE 50+7+8 STRT-SEQ v4 N2IndSeq N2RevSeq2 Lane 1 s_1_1_Indx11.txt 140514_HL166
BL_totoSmartSeq2_1ng-100pg-10pg-RIII-UHRR_Mar05 1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E5 ERCC Smart2_T30VN Smart2_TSO IS Nextera XT S501/N701 PE 50+7+8 HP10 HP12 HP11 Lane 6 s_6_1_N701.txt 140514_HL166
1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E5 ERCC Smart2_T30VN Smart2_TSO IS Nextera XT S502/N702 PE 50+7+8 HP10 HP12 HP11 Lane 6 s_6_1_N702.txt 140514_HL166
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E6 ERCC Smart2_T30VN Smart2_TSO IS Nextera XT S503/N703 PE 50+7+8 HP10 HP12 HP11 Lane 6 s_6_1_N703.txt 140514_HL166
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E6 ERCC Smart2_T30VN Smart2_TSO IS Nextera XT S504/N704 PE 50+7+8 HP10 HP12 HP11 Lane 6 s_6_1_N704.txt 140514_HL166
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min 1:2E7 ERCC Smart2_T30VN Smart2_TSO IS Nextera XT S505/N705 PE 50+7+8 HP10 HP12 HP11 Lane 6 s_6_1_N705.txt 140514_HL166
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min 1:2E7 ERCC Smart2_T30VN Smart2_TSO IS Nextera XT S506/N706 PE 50+7+8 HP10 HP12 HP11 Lane 6 s_6_1_N706.txt 140514_HL166
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min 1:2E7 ERCC Smart2_T30VN Smart2_TSO IS Nextera XT S507/N707 PE 50+7+8 HP10 HP12 HP11 Lane 6 s_6_1_N707.txt 140514_HL166
NTC; 1min 0.05U RIII Frag; Affy. PAP 4min 1:2E7 ERCC Smart2_T30VN Smart2_TSO IS Nextera XT S508/N708 PE 50+7+8 HP10 HP12 HP11 Lane 6 s_6_1_N708.txt 140514_HL166
BL_totoQuartzSeq_1ng-100pg-10pg-RIII-UHRR_Mar05 1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E5 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S501/N709 PE 50+7+8 HP10 HP12 HP11 Lane 7 s_6_1_N709.txt 140514_HL166
1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E5 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S502/N710 PE 50+7+8 HP10 HP12 HP11 Lane 7 s_6_1_N710.txt 140514_HL166
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E6 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S503/N711 PE 50+7+8 HP10 HP12 HP11 Lane 7 s_6_1_N711.txt 140514_HL166
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E6 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S504/N712 PE 50+7+8 HP10 HP12 HP11 Lane 7 s_6_1_N712.txt 140514_HL166
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min 1:2E7 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S505/N701 PE 50+7+8 HP10 HP12 HP11 Lane 7 s_6_1_N701.txt 140514_HL166
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min 1:2E7 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S506/N702 PE 50+7+8 HP10 HP12 HP11 Lane 7 s_6_1_N702.txt 140514_HL166
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min 1:2E7 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S507/N703 PE 50+7+8 HP10 HP12 HP11 Lane 7 s_6_1_N703.txt 140514_HL166
NTC; 1min 0.05U RIII Frag; Affy. PAP 4min 1:2E7 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S508/N704 PE 50+7+8 HP10 HP12 HP11 Lane 7 s_6_1_N704.txt 140514_HL166
BL_totoRNAseq_10x10pg_0.01-0.1URIII_UHRR_Jan22 10x10pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 4min 1:2E7 ERCC T20VN_id1-10 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 81 PE 50+7+8 STRT-SEQ v4 N2IndSeq N2RevSeq2 Lane 2, 8 s_2(8)_1_Indx81.txt 140514_HL166
10x10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min 1:2E7 ERCC T20VN_id1-10 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 82 PE 50+7+8 STRT-SEQ v4 N2IndSeq N2RevSeq2 Lane 2, 8 s_2(8)_1_Indx82.txt 140514_HL166
10x10pg UHRR; 1min 0.01U RIII Frag; Affy. PAP 4min 1:2E7 ERCC T20VN_id1-10 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 83 PE 50+7+8 STRT-SEQ v4 N2IndSeq N2RevSeq2 Lane 2, 8 s_2(8)_1_Indx83.txt 140514_HL166
BL-totoSTRTseq-1ng_100pg_10pg_RIII_UHRR-Mar05 1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E5 ERCC C1-P1-T31 C1-P1-RNA-TSO C1-P1-PCR-2 Sten Tagmentation C1-TN5-01 SE 50+7 C1-P1-PCR-2 C1-TN5-U N/A Lane 1,2 s_1_1_StenTn5-id01.txt /home/kunzhang/seqStore/140701_HL169
1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E5 ERCC C1-P1-T31 C1-P1-RNA-TSO C1-P1-PCR-2 Sten Tagmentation C1-TN5-02 SE 50+7 C1-P1-PCR-2 C1-TN5-U N/A Lane 1,2 s_1_1_StenTn5-id02.txt /home/kunzhang/seqStore/140701_HL169
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E6 ERCC C1-P1-T31 C1-P1-RNA-TSO C1-P1-PCR-2 Sten Tagmentation C1-TN5-03 SE 50+7 C1-P1-PCR-2 C1-TN5-U N/A Lane 1,2 s_1_1_StenTn5-id03.txt /home/kunzhang/seqStore/140701_HL169
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E6 ERCC C1-P1-T31 C1-P1-RNA-TSO C1-P1-PCR-2 Sten Tagmentation C1-TN5-04 SE 50+7 C1-P1-PCR-2 C1-TN5-U N/A Lane 1,2 s_1_1_StenTn5-id04.txt /home/kunzhang/seqStore/140701_HL169
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min 1:2E7 ERCC C1-P1-T31 C1-P1-RNA-TSO C1-P1-PCR-2 Sten Tagmentation C1-TN5-05 SE 50+7 C1-P1-PCR-2 C1-TN5-U N/A Lane 1,2 s_1_1_StenTn5-id05.txt /home/kunzhang/seqStore/140701_HL169
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min 1:2E7 ERCC C1-P1-T31 C1-P1-RNA-TSO C1-P1-PCR-2 Sten Tagmentation C1-TN5-06 SE 50+7 C1-P1-PCR-2 C1-TN5-U N/A Lane 1,2 s_1_1_StenTn5-id06.txt /home/kunzhang/seqStore/140701_HL169
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min 1:2E7 ERCC C1-P1-T31 C1-P1-RNA-TSO C1-P1-PCR-2 Sten Tagmentation C1-TN5-07 SE 50+7 C1-P1-PCR-2 C1-TN5-U N/A Lane 1,2 s_1_1_StenTn5-id07.txt /home/kunzhang/seqStore/140701_HL169
NTC; 1min 0.05U RIII Frag; Affy. PAP 4min 1:2E7 ERCC C1-P1-T31 C1-P1-RNA-TSO C1-P1-PCR-2 Sten Tagmentation C1-TN5-08 SE 50+7 C1-P1-PCR-2 C1-TN5-U N/A Lane 1,2 s_1_1_StenTn5-id08.txt /home/kunzhang/seqStore/140701_HL169
BL-totoQuartzSeq-1ng_11_15cycles_UHRR-Jun06 1ng UHRR; 1min 0.1U RIII Frag; Affy.PAP, 11cycles 1:2E5 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S503/N701 SE 50+7 NeteraXT_R1 NeteraXT_i7Ind n/a Lane 6 s_6_1_N701.txt /home/kunzhang/seqStore/140701_HL169
1ng UHRR; 1min 0.1U RIII Frag; Affy.PAP, 11cycles 1:2E5 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S503/N702 SE 50+7 NeteraXT_R1 NeteraXT_i7Ind n/a Lane 6 s_6_1_N702.txt /home/kunzhang/seqStore/140701_HL169
1ng UHRR; 1min 0.1U RIII Frag; Affy.PAP, 13cycles 1:2E5 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S503/N703 SE 50+7 NeteraXT_R1 NeteraXT_i7Ind n/a Lane 6 s_6_1_N703.txt /home/kunzhang/seqStore/140701_HL169
1ng UHRR; 1min 0.1U RIII Frag; Affy.PAP, 13cycles 1:2E5 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S503/N704 SE 50+7 NeteraXT_R1 NeteraXT_i7Ind n/a Lane 6 s_6_1_N704.txt /home/kunzhang/seqStore/140701_HL169
1ng UHRR; 1min 0.1U RIII Frag; Affy.PAP, 15cycles 1:2E5 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S503/N705 SE 50+7 NeteraXT_R1 NeteraXT_i7Ind n/a Lane 6 s_6_1_N705.txt /home/kunzhang/seqStore/140701_HL169
1ng UHRR; 1min 0.1U RIII Frag; Affy.PAP, 15cycles 1:2E5 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S503/N706 SE 50+7 NeteraXT_R1 NeteraXT_i7Ind n/a Lane 6 s_6_1_N706.txt /home/kunzhang/seqStore/140701_HL169
NTC; 1min 0.1U RIII Frag; Affy.PAP, 15cycles 1:2E5 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S503/N707 SE 50+7 NeteraXT_R1 NeteraXT_i7Ind n/a Lane 6 s_6_1_N707.txt /home/kunzhang/seqStore/140701_HL169
NTC; 1min 0.1U RIII Frag; Affy.PAP, 15cycles 1:2E5 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S503/N708 SE 50+7 NeteraXT_R1 NeteraXT_i7Ind n/a Lane 6 s_6_1_N708.txt /home/kunzhang/seqStore/140701_HL169
1ng UHRR; Mg Frag; Affy.PAP, 15cycles 1:2E5 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S503/N709 SE 50+7 NeteraXT_R1 NeteraXT_i7Ind n/a Lane 6 s_6_1_N709.txt /home/kunzhang/seqStore/140701_HL169
1ng UHRR; Mg Frag; Affy.PAP, 15cycles 1:2E5 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S503/N710 SE 50+7 NeteraXT_R1 NeteraXT_i7Ind n/a Lane 6 s_6_1_N7010.txt /home/kunzhang/seqStore/140701_HL169
NTC; Mg Frag; Affy.PAP, 15cycles 1:2E5 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S503/N711 SE 50+7 NeteraXT_R1 NeteraXT_i7Ind n/a Lane 6 s_6_1_N7011.txt /home/kunzhang/seqStore/140701_HL169
NTC; Mg Frag; Affy.PAP, 15cycles 1:2E5 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S503/N712 SE 50+7 NeteraXT_R1 NeteraXT_i7Ind n/a Lane 6 s_6_1_N7012.txt /home/kunzhang/seqStore/140701_HL169