Blue:RNA-Seq Libraries:2014
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Library | Condition | RNA Spike-in | T20-30 (Barcode) | TSO | PCR Primers | Library Processing | N2/Nextera Index | Run Type, Minmum BP | Read 1 Sequencing Primer | Index Primer | Read 2 Sequencing Primer | Seq Lane | File | File Location |
BL_totoRNAseq_1ng-100pg-Mg-UHRR_Mar05 | 1ng UHRR; 2min Mg Frag; Enz. PAP 5min | 1:2E5 ERCC | T20VN_id1 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 1 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | Lane 5 | s_5_1_Indx01.txt | 140514_HL166 |
1ng UHRR; 2min Mg Frag; Enz. PAP 5min | 1:2E5 ERCC | T20VN_id1 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 2 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | Lane 5 | s_5_1_Indx02.txt | 140514_HL166 | |
100pg UHRR; 2min Mg Frag; Enz. PAP 5min | 1:2E6 ERCC | T20VN_id1 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 3 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | Lane 5 | s_5_1_Indx03.txt | 140514_HL166 | |
100pg UHRR; 2min Mg Frag; Enz. PAP 5min | 1:2E6 ERCC | T20VN_id1 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 4 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | Lane 5 | s_5_1_Indx04.txt | 140514_HL166 | |
NTC; 2min Mg Frag; Enz. PAP 5min | 1:2E7 ERCC | T20VN_id11 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 6 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | Lane 5 | s_5_1_Indx06.txt | 140514_HL166 | |
BL_totoRNAseq_1ng-100pg-RIII-UHRR_Mar05 | 1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E5 ERCC | T20VN_id1 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 7 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | Lane 5 | s_5_1_Indx07.txt | 140514_HL166 |
1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E5 ERCC | T20VN_id1 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 8 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | Lane 5 | s_5_1_Indx08.txt | 140514_HL166 | |
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E6 ERCC | T20VN_id1 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 9 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | Lane 5 | s_5_1_Indx09.txt | 140514_HL166 | |
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E6 ERCC | T20VN_id1 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 10 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | Lane 5 | s_5_1_Indx10.txt | 140514_HL166 | |
NTC; 1min 0.1U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | T20VN_id11 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 12 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | Lane 5 | s_5_1_Indx12.txt | 140514_HL166 | |
BL_totoRNAseq_10x10pg-Mg-UHRR_Mar05 | 10x10pg UHRR; 2min Mg Frag; Enz. PAP 5min | 1:2E7 ERCC | T20VN_id1-10 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 5 | PE 50+7+8 | STRT-SEQ v4 | N2IndSeq | N2RevSeq2 | Lane 5 | s_5_1_Indx05.txt | 140514_HL166 |
BL_totoRNAseq_10x10pg-RIII-UHRR_Mar05 | 10x10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | T20VN_id1-10 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 11 | PE 50+7+8 | STRT-SEQ v4 | N2IndSeq | N2RevSeq2 | Lane 1 | s_1_1_Indx11.txt | 140514_HL166 |
BL_totoSmartSeq2_1ng-100pg-10pg-RIII-UHRR_Mar05 | 1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E5 ERCC | Smart2_T30VN | Smart2_TSO | IS | Nextera XT | S501/N701 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 6 | s_6_1_N701.txt | 140514_HL166 |
1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E5 ERCC | Smart2_T30VN | Smart2_TSO | IS | Nextera XT | S502/N702 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 6 | s_6_1_N702.txt | 140514_HL166 | |
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E6 ERCC | Smart2_T30VN | Smart2_TSO | IS | Nextera XT | S503/N703 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 6 | s_6_1_N703.txt | 140514_HL166 | |
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E6 ERCC | Smart2_T30VN | Smart2_TSO | IS | Nextera XT | S504/N704 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 6 | s_6_1_N704.txt | 140514_HL166 | |
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | Smart2_T30VN | Smart2_TSO | IS | Nextera XT | S505/N705 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 6 | s_6_1_N705.txt | 140514_HL166 | |
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | Smart2_T30VN | Smart2_TSO | IS | Nextera XT | S506/N706 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 6 | s_6_1_N706.txt | 140514_HL166 | |
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | Smart2_T30VN | Smart2_TSO | IS | Nextera XT | S507/N707 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 6 | s_6_1_N707.txt | 140514_HL166 | |
NTC; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | Smart2_T30VN | Smart2_TSO | IS | Nextera XT | S508/N708 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 6 | s_6_1_N708.txt | 140514_HL166 | |
BL_totoQuartzSeq_1ng-100pg-10pg-RIII-UHRR_Mar05 | 1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S501/N709 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 7 | s_6_1_N709.txt | 140514_HL166 |
1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S502/N710 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 7 | s_6_1_N710.txt | 140514_HL166 | |
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E6 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N711 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 7 | s_6_1_N711.txt | 140514_HL166 | |
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E6 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S504/N712 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 7 | s_6_1_N712.txt | 140514_HL166 | |
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S505/N701 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 7 | s_6_1_N701.txt | 140514_HL166 | |
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S506/N702 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 7 | s_6_1_N702.txt | 140514_HL166 | |
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S507/N703 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 7 | s_6_1_N703.txt | 140514_HL166 | |
NTC; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S508/N704 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 7 | s_6_1_N704.txt | 140514_HL166 | |
BL_totoRNAseq_10x10pg_0.01-0.1URIII_UHRR_Jan22 | 10x10pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | T20VN_id1-10 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 81 | PE 50+7+8 | STRT-SEQ v4 | N2IndSeq | N2RevSeq2 | Lane 2, 8 | s_2(8)_1_Indx81.txt | 140514_HL166 |
10x10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | T20VN_id1-10 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 82 | PE 50+7+8 | STRT-SEQ v4 | N2IndSeq | N2RevSeq2 | Lane 2, 8 | s_2(8)_1_Indx82.txt | 140514_HL166 | |
10x10pg UHRR; 1min 0.01U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | T20VN_id1-10 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 83 | PE 50+7+8 | STRT-SEQ v4 | N2IndSeq | N2RevSeq2 | Lane 2, 8 | s_2(8)_1_Indx83.txt | 140514_HL166 | |
BL-totoSTRTseq-1ng_100pg_10pg_RIII_UHRR-Mar05 | 1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E5 ERCC | C1-P1-T31 | C1-P1-RNA-TSO | C1-P1-PCR-2 | Sten Tagmentation | C1-TN5-01 | SE 50+7 | C1-P1-PCR-2 | C1-TN5-U | N/A | Lane 1,2 | s_1_1_StenTn5-id01.txt | /home/kunzhang/seqStore/140701_HL169 |
1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E5 ERCC | C1-P1-T31 | C1-P1-RNA-TSO | C1-P1-PCR-2 | Sten Tagmentation | C1-TN5-02 | SE 50+7 | C1-P1-PCR-2 | C1-TN5-U | N/A | Lane 1,2 | s_1_1_StenTn5-id02.txt | /home/kunzhang/seqStore/140701_HL169 | |
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E6 ERCC | C1-P1-T31 | C1-P1-RNA-TSO | C1-P1-PCR-2 | Sten Tagmentation | C1-TN5-03 | SE 50+7 | C1-P1-PCR-2 | C1-TN5-U | N/A | Lane 1,2 | s_1_1_StenTn5-id03.txt | /home/kunzhang/seqStore/140701_HL169 | |
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E6 ERCC | C1-P1-T31 | C1-P1-RNA-TSO | C1-P1-PCR-2 | Sten Tagmentation | C1-TN5-04 | SE 50+7 | C1-P1-PCR-2 | C1-TN5-U | N/A | Lane 1,2 | s_1_1_StenTn5-id04.txt | /home/kunzhang/seqStore/140701_HL169 | |
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | C1-P1-T31 | C1-P1-RNA-TSO | C1-P1-PCR-2 | Sten Tagmentation | C1-TN5-05 | SE 50+7 | C1-P1-PCR-2 | C1-TN5-U | N/A | Lane 1,2 | s_1_1_StenTn5-id05.txt | /home/kunzhang/seqStore/140701_HL169 | |
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | C1-P1-T31 | C1-P1-RNA-TSO | C1-P1-PCR-2 | Sten Tagmentation | C1-TN5-06 | SE 50+7 | C1-P1-PCR-2 | C1-TN5-U | N/A | Lane 1,2 | s_1_1_StenTn5-id06.txt | /home/kunzhang/seqStore/140701_HL169 | |
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | C1-P1-T31 | C1-P1-RNA-TSO | C1-P1-PCR-2 | Sten Tagmentation | C1-TN5-07 | SE 50+7 | C1-P1-PCR-2 | C1-TN5-U | N/A | Lane 1,2 | s_1_1_StenTn5-id07.txt | /home/kunzhang/seqStore/140701_HL169 | |
NTC; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | C1-P1-T31 | C1-P1-RNA-TSO | C1-P1-PCR-2 | Sten Tagmentation | C1-TN5-08 | SE 50+7 | C1-P1-PCR-2 | C1-TN5-U | N/A | Lane 1,2 | s_1_1_StenTn5-id08.txt | /home/kunzhang/seqStore/140701_HL169 | |
BL-totoQuartzSeq-1ng_11_15cycles_UHRR-Jun06 | 1ng UHRR; 1min 0.1U RIII Frag; Affy.PAP, 11cycles | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N701 | SE 50+7 | NeteraXT_R1 | NeteraXT_i7Ind | n/a | Lane 6 | s_6_1_N701.txt | /home/kunzhang/seqStore/140701_HL169 |
1ng UHRR; 1min 0.1U RIII Frag; Affy.PAP, 11cycles | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N702 | SE 50+7 | NeteraXT_R1 | NeteraXT_i7Ind | n/a | Lane 6 | s_6_1_N702.txt | /home/kunzhang/seqStore/140701_HL169 | |
1ng UHRR; 1min 0.1U RIII Frag; Affy.PAP, 13cycles | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N703 | SE 50+7 | NeteraXT_R1 | NeteraXT_i7Ind | n/a | Lane 6 | s_6_1_N703.txt | /home/kunzhang/seqStore/140701_HL169 | |
1ng UHRR; 1min 0.1U RIII Frag; Affy.PAP, 13cycles | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N704 | SE 50+7 | NeteraXT_R1 | NeteraXT_i7Ind | n/a | Lane 6 | s_6_1_N704.txt | /home/kunzhang/seqStore/140701_HL169 | |
1ng UHRR; 1min 0.1U RIII Frag; Affy.PAP, 15cycles | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N705 | SE 50+7 | NeteraXT_R1 | NeteraXT_i7Ind | n/a | Lane 6 | s_6_1_N705.txt | /home/kunzhang/seqStore/140701_HL169 | |
1ng UHRR; 1min 0.1U RIII Frag; Affy.PAP, 15cycles | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N706 | SE 50+7 | NeteraXT_R1 | NeteraXT_i7Ind | n/a | Lane 6 | s_6_1_N706.txt | /home/kunzhang/seqStore/140701_HL169 | |
NTC; 1min 0.1U RIII Frag; Affy.PAP, 15cycles | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N707 | SE 50+7 | NeteraXT_R1 | NeteraXT_i7Ind | n/a | Lane 6 | s_6_1_N707.txt | /home/kunzhang/seqStore/140701_HL169 | |
NTC; 1min 0.1U RIII Frag; Affy.PAP, 15cycles | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N708 | SE 50+7 | NeteraXT_R1 | NeteraXT_i7Ind | n/a | Lane 6 | s_6_1_N708.txt | /home/kunzhang/seqStore/140701_HL169 | |
1ng UHRR; Mg Frag; Affy.PAP, 15cycles | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N709 | SE 50+7 | NeteraXT_R1 | NeteraXT_i7Ind | n/a | Lane 6 | s_6_1_N709.txt | /home/kunzhang/seqStore/140701_HL169 | |
1ng UHRR; Mg Frag; Affy.PAP, 15cycles | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N710 | SE 50+7 | NeteraXT_R1 | NeteraXT_i7Ind | n/a | Lane 6 | s_6_1_N7010.txt | /home/kunzhang/seqStore/140701_HL169 | |
NTC; Mg Frag; Affy.PAP, 15cycles | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N711 | SE 50+7 | NeteraXT_R1 | NeteraXT_i7Ind | n/a | Lane 6 | s_6_1_N7011.txt | /home/kunzhang/seqStore/140701_HL169 | |
NTC; Mg Frag; Affy.PAP, 15cycles | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N712 | SE 50+7 | NeteraXT_R1 | NeteraXT_i7Ind | n/a | Lane 6 | s_6_1_N7012.txt | /home/kunzhang/seqStore/140701_HL169 |