Blue:RNAseq Primer List

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Adaptor Structure[edit]

Rui's totoRNAseq Page


totoRNA 5' primer sequence[edit]

  • The 5' TSO adaptors list
Tube ID Primer full name Primer full sequence PE_F-N10 Barcode TSO
TSO.r04 iso_B_TSO_N10 iso-dCiso-dGiso-dCACACTCTTTCCCTACACGACGNNNUNNNNUNNNrGrGrG /5Me-isodC//iisodG//iMe-isodC/ACACTCTTTCCCTACACGACGNNN/ideoxyU/NNNN/ideoxyU/NNN \ rGrGrG
TSO.r05 IsoL_B_TSO_N8 T-iSp18-ACACTCTTTCCCTACACGACGNNNNUNNNNrGrG+G T/iSp18/ACACTCTTTCCCTACACGACGNNNN/ideoxyU/NNNNrGrG+G \ rGrG+G
TSO.r04_N6 iso_B_TSO_N6 isodC-isodG-isodC-ACACTCTTTCCCTACACGACGNNNUNNNrGrGrG /5Me-isodC//iisodG//iMe-isodC/ACACTCTTTCCCTACACGACGNNN/ideoxyU/NNNrGrGrG \ rGrGrG
TSO.r06 iso_SMARTer II A isodC-isodG-isodC-AAGCAGTGGTATCAACGCAGAGTACrGrGrG /5Me-isodC//iisodG//iMe-isodC/AAGCAGTGGTATCAACGCAGAGTACrGrGrG \ rGrGrG
PB_PCR.F PB_PCR.F AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG \
PB_PCR.F* PB_PCR.Fmod AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG A*A*T*GATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG \
P1-STRT P1-STRT AATGATACGGCGACCACCGAGATCTAAGCAGTGGTATCAACGCAGAGT \
ILMN_PCR.F* ILMN_PCR.Fmod AATGATACGGCGACCACCGAGATC A*A*T*GATACGGCGACCACCGAGATC \

totoRNA 3' primer sequence[edit]

  • The 3' T20 adaptors list
Tube ID Primer full name Primer full sequence PE_R Barcode T20V
T20VN.r01 T20VN_PE_R /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT TTTTTTTTTTTTTTTTTTTTVN
T20V.id01 T20V.BC8.PE_R.Indx1 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCTGAGACTTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT TCTGAGAC TTTTTTTTTTTTTTTTTTTTV
T20V.id02 T20V.BC8.PE_R.Indx2 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGCCCAAGTTTTTTTTTTTTTTTTTTTTV TGCCCAAG
T20V.id03 T20V.BC8.PE_R.Indx3 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGACTCGCTTTTTTTTTTTTTTTTTTTTTV GACTCGCT
T20V.id04 T20V.BC8.PE_R.Indx4 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCACTCCATTTTTTTTTTTTTTTTTTTTV CCACTCCA
T20V.id05 T20V.BC8.PE_R.Indx5 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAACAGGGTTTTTTTTTTTTTTTTTTTTV GAACAGGG
T20V.id06 T20V.BC8.PE_R.Indx6 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTGCTGATTTTTTTTTTTTTTTTTTTTTV CTGCTGAT
T20V.id07 T20V.BC8.PE_R.Indx7 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTAGTATCTTTTTTTTTTTTTTTTTTTTV CTAGTATC
T20V.id08 T20V.BC8.PE_R.Indx8 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGCTGAAATTTTTTTTTTTTTTTTTTTTV GGCTGAAA
T20V.id09 T20V.BC8.PE_R.Indx9 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAGTGCTTTTTTTTTTTTTTTTTTTTTTV GAGTGCTT
T20V.id10 T20V.BC8.PE_R.Indx10 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATACGCCATTTTTTTTTTTTTTTTTTTTV ATACGCCA
T20V.id11 T20V.BC8.PE_R.Indx11 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGCGTTATTTTTTTTTTTTTTTTTTTTV ATGCGTTA
T20V.id12 T20V.BC8.PE_R.Indx12 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCACCCTTTTTTTTTTTTTTTTTTTTTTV TCACCCTT
T20V.id13 T20V.BC8.PE_R.Indx13 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCAACCGCTTTTTTTTTTTTTTTTTTTTV CCAACCGC
T20V.id14 T20V.BC8.PE_R.Indx14 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGATTACCTTTTTTTTTTTTTTTTTTTTV GGATTACC
T20V.id15 T20V.BC8.PE_R.Indx15 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAGAGACATTTTTTTTTTTTTTTTTTTTV CAGAGACA
T20V.id16 T20V.BC8.PE_R.Indx16 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAATAGTGTTTTTTTTTTTTTTTTTTTTV CAATAGTG
T20V.id17 T20V.BC8.PE_R.Indx17 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGTGGCCATTTTTTTTTTTTTTTTTTTTV GGTGGCCA
T20V.id18 T20V.BC8.PE_R.Indx18 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGAGGGAGTTTTTTTTTTTTTTTTTTTTV GGAGGGAG
T20V.id19 T20V.BC8.PE_R.Indx19 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTTGTCCTTTTTTTTTTTTTTTTTTTTTV GTTGTCCT
T20V.id20 T20V.BC8.PE_R.Indx20 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTCGACCTTTTTTTTTTTTTTTTTTTTTV TTCGACC
T20V.id21 T20V.BC8.PE_R.Indx21 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTTATTGTTTTTTTTTTTTTTTTTTTTV GCTTATTG
T20V.id22 T20V.BC8.PE_R.Indx22 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAACCTTTTTTTTTTTTTTTTTTTTTTTV CAACCTTT
T20V.id23 T20V.BC8.PE_R.Indx23 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTTAGGTATTTTTTTTTTTTTTTTTTTTV CTTAGGTA
T20V.id24 T20V.BC8.PE_R.Indx24 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTCACCATTTTTTTTTTTTTTTTTTTTV GCTCACCA
T20V.id25 T20V.BC8.PE_R.Indx25 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGAAATGCTTTTTTTTTTTTTTTTTTTTV AGAAATGC
T20V.id26 T20V.BC8.PE_R.Indx26 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTTACTGATTTTTTTTTTTTTTTTTTTTV TACTGATT
T20V.id27 T20V.BC8.PE_R.Indx27 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTCACGGGTTTTTTTTTTTTTTTTTTTTV CTCACGGG
T20V.id28 T20V.BC8.PE_R.Indx28 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCACTGTCATTTTTTTTTTTTTTTTTTTTV
T20V.id29 T20V.BC8.PE_R.Indx29 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTCTTGGGTTTTTTTTTTTTTTTTTTTTV
T20V.id30 T20V.BC8.PE_R.Indx30 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTACCGGCTTTTTTTTTTTTTTTTTTTTTTV
T20V.id31 T20V.BC8.PE_R.Indx31 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTAACCACATTTTTTTTTTTTTTTTTTTTV
T20V.id32 T20V.BC8.PE_R.Indx32 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTACGCGCGTTTTTTTTTTTTTTTTTTTTV
T20V.id33 T20V.BC8.PE_R.Indx33 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCTCGATGTTTTTTTTTTTTTTTTTTTTV
T20V.id34 T20V.BC8.PE_R.Indx34 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGAGCTCATTTTTTTTTTTTTTTTTTTTV
T20V.id35 T20V.BC8.PE_R.Indx35 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTATGGTCCTTTTTTTTTTTTTTTTTTTTV
T20V.id36 T20V.BC8.PE_R.Indx36 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGTCCGGCTTTTTTTTTTTTTTTTTTTTV
T20V.id37 T20V.BC8.PE_R.Indx37 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAAGGAATCTTTTTTTTTTTTTTTTTTTTV
T20V.id38 T20V.BC8.PE_R.Indx38 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCATCGTAGTTTTTTTTTTTTTTTTTTTTV
T20V.id39 T20V.BC8.PE_R.Indx39 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGTTGAGTTTTTTTTTTTTTTTTTTTTV
T20V.id40 T20V.BC8.PE_R.Indx40 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGTATCCTTTTTTTTTTTTTTTTTTTTV
T20V.id41 T20V.BC8.PE_R.Indx41 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGACAGGTTTTTTTTTTTTTTTTTTTTTV
T20V.id42 T20V.BC8.PE_R.Indx42 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTACGCCAATTTTTTTTTTTTTTTTTTTTV
T20V.id43 T20V.BC8.PE_R.Indx43 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAAGGCAATTTTTTTTTTTTTTTTTTTTV
T20V.id44 T20V.BC8.PE_R.Indx44 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCACCCGAGTTTTTTTTTTTTTTTTTTTTV
T20V.id45 T20V.BC8.PE_R.Indx45 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGTGTGTCTTTTTTTTTTTTTTTTTTTTV
T20V.id46 T20V.BC8.PE_R.Indx46 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCTTGTGCTTTTTTTTTTTTTTTTTTTTV
T20V.id47 T20V.BC8.PE_R.Indx47 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGTACCTGTTTTTTTTTTTTTTTTTTTTV
T20V.id48 T20V.BC8.PE_R.Indx48 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGCGTCCTTTTTTTTTTTTTTTTTTTTTV
T20V.id49 T20V.BC8.PE_R.Indx49 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGAACTGTTTTTTTTTTTTTTTTTTTTTV
T20V.id50 T20V.BC8.PE_R.Indx50 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAATGCTCTTTTTTTTTTTTTTTTTTTTV
T20V.id51 T20V.BC8.PE_R.Indx51 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTTAGGCGTTTTTTTTTTTTTTTTTTTTV
T20V.id52 T20V.BC8.PE_R.Indx52 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTAGACGCTTTTTTTTTTTTTTTTTTTTTV
T20V.id53 T20V.BC8.PE_R.Indx53 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTGGTGTGTTTTTTTTTTTTTTTTTTTTV
T20V.id54 T20V.BC8.PE_R.Indx54 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGCGGTAATTTTTTTTTTTTTTTTTTTTV
T20V.id55 T20V.BC8.PE_R.Indx55 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCTTAAGTTTTTTTTTTTTTTTTTTTTTV
T20V.id56 T20V.BC8.PE_R.Indx56 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCGGTTTCTTTTTTTTTTTTTTTTTTTTV
T20V.id57 T20V.BC8.PE_R.Indx57 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAAGCCTAGTTTTTTTTTTTTTTTTTTTTV
T20V.id58 T20V.BC8.PE_R.Indx58 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTAGCCAGGTTTTTTTTTTTTTTTTTTTTV
T20V.id59 T20V.BC8.PE_R.Indx59 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGGTAAATTTTTTTTTTTTTTTTTTTTV
T20V.id60 T20V.BC8.PE_R.Indx60 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCACATAGGTTTTTTTTTTTTTTTTTTTTV
T20V.id61 T20V.BC8.PE_R.Indx61 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCCGGTGATTTTTTTTTTTTTTTTTTTTV
T20V.id62 T20V.BC8.PE_R.Indx62 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCTAAACCTTTTTTTTTTTTTTTTTTTTV
T20V.id63 T20V.BC8.PE_R.Indx63 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCGTGTCCCTTTTTTTTTTTTTTTTTTTTV
T20V.id64 T20V.BC8.PE_R.Indx64 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGGGTAGGTTTTTTTTTTTTTTTTTTTTV
T20V.id65 T20V.BC8.PE_R.Indx65 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGGAGATGTTTTTTTTTTTTTTTTTTTTV
T20V.id66 T20V.BC8.PE_R.Indx66 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGGAAGTATTTTTTTTTTTTTTTTTTTTV
T20V.id67 T20V.BC8.PE_R.Indx67 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGGCATGCTTTTTTTTTTTTTTTTTTTTV
T20V.id68 T20V.BC8.PE_R.Indx68 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTAGGTACTTTTTTTTTTTTTTTTTTTTV
T20V.id69 T20V.BC8.PE_R.Indx69 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTGGATTTTTTTTTTTTTTTTTTTTTTV
T20V.id70 T20V.BC8.PE_R.Indx70 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGCTCCCTTTTTTTTTTTTTTTTTTTTTV
T20V.id71 T20V.BC8.PE_R.Indx71 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAAGCGCCCTTTTTTTTTTTTTTTTTTTTV
T20V.id72 T20V.BC8.PE_R.Indx72 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCGACGGATTTTTTTTTTTTTTTTTTTTTV
T20V.id73 T20V.BC8.PE_R.Indx73 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGATAGCGTTTTTTTTTTTTTTTTTTTTV
T20V.id74 T20V.BC8.PE_R.Indx74 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCCCAGTGTTTTTTTTTTTTTTTTTTTTV
T20V.id75 T20V.BC8.PE_R.Indx75 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGAAGACTTTTTTTTTTTTTTTTTTTTV
T20V.id76 T20V.BC8.PE_R.Indx76 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAATCAGGATTTTTTTTTTTTTTTTTTTTV
T20V.id77 T20V.BC8.PE_R.Indx77 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGCTTCTTTTTTTTTTTTTTTTTTTTTV
T20V.id78 T20V.BC8.PE_R.Indx78 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCAAGCAGTTTTTTTTTTTTTTTTTTTTV
T20V.id79 T20V.BC8.PE_R.Indx79 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAAGTCGTTTTTTTTTTTTTTTTTTTTTV
T20V.id80 T20V.BC8.PE_R.Indx80 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCGCCGCATTTTTTTTTTTTTTTTTTTTV
T20V.id81 T20V.BC8.PE_R.Indx81 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCAGTTACTTTTTTTTTTTTTTTTTTTTV
T20V.id82 T20V.BC8.PE_R.Indx82 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTAACGTGCTTTTTTTTTTTTTTTTTTTTV
T20V.id83 T20V.BC8.PE_R.Indx83 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGTAGGATTTTTTTTTTTTTTTTTTTTTV
T20V.id84 T20V.BC8.PE_R.Indx84 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGCACATCTTTTTTTTTTTTTTTTTTTTV
T20V.id85 T20V.BC8.PE_R.Indx85 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGTCTTGTTTTTTTTTTTTTTTTTTTTV
T20V.id86 T20V.BC8.PE_R.Indx86 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGCAACCATTTTTTTTTTTTTTTTTTTTV
T20V.id87 T20V.BC8.PE_R.Indx87 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCACTTGTCTTTTTTTTTTTTTTTTTTTTV
T20V.id88 T20V.BC8.PE_R.Indx88 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAGTTTGGTTTTTTTTTTTTTTTTTTTTV
T20V.id89 T20V.BC8.PE_R.Indx89 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGCTGGATTTTTTTTTTTTTTTTTTTTV
T20V.id90 T20V.BC8.PE_R.Indx90 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAGAGCGCTTTTTTTTTTTTTTTTTTTTV
T20V.id91 T20V.BC8.PE_R.Indx91 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCGAACATTTTTTTTTTTTTTTTTTTTTV
T20V.id92 T20V.BC8.PE_R.Indx92 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTACTATGCGTTTTTTTTTTTTTTTTTTTTV
T20V.id93 T20V.BC8.PE_R.Indx93 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTAGCACTTTTTTTTTTTTTTTTTTTTTV
T20V.id94 T20V.BC8.PE_R.Indx94 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGTCCATTTTTTTTTTTTTTTTTTTTTV
T20V.id95 T20V.BC8.PE_R.Indx95 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCCTGCCTTTTTTTTTTTTTTTTTTTTTV
T20V.id96 T20V.BC8.PE_R.Indx96 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTGTCAGCTTTTTTTTTTTTTTTTTTTTV
N2.id1 PCR_R.N2Ind1 CAAGCAGAAGACGGCATACGAGATCGTGATCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CGTGAT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id2 PCR_R.N2Ind2 CAAGCAGAAGACGGCATACGAGATACATCGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT ACATCG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id3 PCR_R.N2Ind3 CAAGCAGAAGACGGCATACGAGATGCCTAACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT GCCTAA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id4 PCR_R.N2Ind4 CAAGCAGAAGACGGCATACGAGATTGGTCACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT TGGTCA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id5 PCR_R.N2Ind5 CAAGCAGAAGACGGCATACGAGATCACTGTCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CACTGT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id6 PCR_R.N2Ind6 CAAGCAGAAGACGGCATACGAGATATTGGCCTCGGCATTCCTGCTGAACCGCTCT CAAGCAGAAGACGGCATACGAGAT ATTGGC CTCGGCATTCCTGCTGAACCGCTCTT
N2.id7 PCR_R.N2Ind7 CAAGCAGAAGACGGCATACGAGATGATCTGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT GATCTG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id8 PCR_R.N2Ind8 CAAGCAGAAGACGGCATACGAGATTCAAGTCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT TCAAGT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id9 PCR_R.N2Ind9 CAAGCAGAAGACGGCATACGAGATCTGATCCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CTGATC CTCGGCATTCCTGCTGAACCGCTCTT
N2.id10 PCR_R.N2Ind10 CAAGCAGAAGACGGCATACGAGATAAGCTACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT AAGCTA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id11 PCR_R.N2Ind11 CAAGCAGAAGACGGCATACGAGATGTAGCCCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT GTAGCC CTCGGCATTCCTGCTGAACCGCTCTT
N2.id12 PCR_R.N2Ind12 CAAGCAGAAGACGGCATACGAGATTACAAGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT TACAAG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id13 PCR_R.N2Ind13 CAAGCAGAAGACGGCATACGAGAT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id14 PCR_R.N2Ind14 CAAGCAGAAGACGGCATACGAGAT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id81 PCR_R.N2Ind81 CAAGCAGAAGACGGCATACGAGATCCAGGTCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CCAGGT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id82 PCR_R.N2Ind82 CAAGCAGAAGACGGCATACGAGATCGTTTGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CGTTTG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id83 PCR_R.N2Ind83 CAAGCAGAAGACGGCATACGAGATGACCACCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT GACCAC CTCGGCATTCCTGCTGAACCGCTCTT
N2.id84 PCR_R.N2Ind84 CAAGCAGAAGACGGCATACGAGATACAAGACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT ACAAGA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id85 PCR_R.N2Ind85 CAAGCAGAAGACGGCATACGAGATACCGCACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT ACCGCA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id86 PCR_R.N2Ind86 CAAGCAGAAGACGGCATACGAGATTGGGTACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT TGGGTA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id87 PCR_R.N2Ind87 CAAGCAGAAGACGGCATACGAGATATTCCGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT ATTCCG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id88 PCR_R.N2Ind88 CAAGCAGAAGACGGCATACGAGATGAATGTCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT GAATGT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id89 PCR_R.N2Ind89 CAAGCAGAAGACGGCATACGAGATGCTGATCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT GCTGAT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id90 PCR_R.N2Ind90 CAAGCAGAAGACGGCATACGAGATAGTGCTCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT AGTGCT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id91 PCR_R.N2Ind91 CAAGCAGAAGACGGCATACGAGATCAGGGACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CAGGGA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id92 PCR_R.N2Ind92 CAAGCAGAAGACGGCATACGAGATCATGCGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CATGCG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id93 PCR_R.N2Ind93 CAAGCAGAAGACGGCATACGAGATTGCCTACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT TGCCTA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id94 PCR_R.N2Ind94 CAAGCAGAAGACGGCATACGAGATCTATACCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CTATAC CTCGGCATTCCTGCTGAACCGCTCTT
N2.id95 PCR_R.N2Ind95 CAAGCAGAAGACGGCATACGAGATCCGAGTCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CCGAGT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id96 PCR_R.N2Ind96 CAAGCAGAAGACGGCATACGAGATACCTGCCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT ACCTGC CTCGGCATTCCTGCTGAACCGCTCTT
PGR_PCR_R CAAGCAGAAGACGGCATACGAGATCTCGGCATTCCTGCTGAACCGCTCTT CGAGAT
PGR_PCR_Rb PGR_PCR_Rmod CAAGCAGAAGACGGCATACGAGATCTCGGCATTCCTGCTGAACCGCTCTT /5Phos/CAAGCAGAAGACGGCATACGAGATCTCGGCATTCCTGCTGAACCGCTCTT
ILMN_PCR_Rb ILMN_PCR_Rmod CAAGCAGAAGACGGCATACGAGAT /5Phos/CAAGCAGAAGACGGCATACGAGAT

IVT 5' primer sequence[edit]

  • The 5' adaptors list
Primer full name Primer full sequence PB_PCR_F Complementary Seq Random Sequence
RL.IVT.N6 ACACTCTTTCCCTACACGACGNNNNNN ACACTCTTTCCCTACACGACG NNNNNN
RL.IVT.N9 ACACTCTTTCCCTACACGACGNNNNNNNNN ACACTCTTTCCCTACACGACG NNNNNNNNN


IVT 3' primer sequence[edit]

  • The 3' adaptors list
Primer full name Primer full sequence T20 Complementary Seq Novel T7 Seq
RL.Add.T7 CTTTAATTATGCTGAGTGATATCCCCGTAAGGACGACTTGGCGAGAA GCATTCCTGCTGAACCGCTCTT CTTTAATTATGCTGAGTGATATCCC


MARS-Seq/STRT-Seq/Smart-Seq2/Quartz-Seq Primer Sequences[edit]

Protocol Primer full name Primer full sequence Modification Barcode Seq
MARS-Seq MARS_T20N_id01 CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTCGTGATNNNNTTTTTTTTTTTTTTTTTTTTN N/A CGTGAT
MARS-Seq MARS_T20N_id02 CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTACATCGNNNNTTTTTTTTTTTTTTTTTTTTN N/A ACATCG
MARS-Seq MARS_T20N_id03 CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTGCCTAANNNNTTTTTTTTTTTTTTTTTTTTN N/A GCCTAA
MARS-Seq MARS_T20N_id04 CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTTGGTCANNNNTTTTTTTTTTTTTTTTTTTTN N/A TGGTCA
MARS-Seq MARS_T20N_id05 CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTCACTGTNNNNTTTTTTTTTTTTTTTTTTTTN N/A CACTGT
MARS-Seq MARS_T20N_id06 CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTATTGGCNNNNTTTTTTTTTTTTTTTTTTTTN N/A ATTGGC
MARS-Seq MARS_T20N_id07 CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTGATCTGNNNNTTTTTTTTTTTTTTTTTTTTN N/A GATCTG
MARS-Seq MARS_T20N_id08 CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTTCAAGTNNNNTTTTTTTTTTTTTTTTTTTTN N/A TCAAGT
MARS-Seq MARS_T20N_id09 CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTCTGATCNNNNTTTTTTTTTTTTTTTTTTTTN N/A CTGATC
MARS-Seq MARS_T20N_id10 CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTAAGCTANNNNTTTTTTTTTTTTTTTTTTTTN N/A AAGCTA
MARS-Seq MARS_RT_2 TCTAGCCTTCTCGCAGCACATC N/A
MARS-Seq P5_Rd1_PCR_F AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT N/A
MARS-Seq P7_Rd2_PCR_R CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT N/A
MARS-Seq MARS_lig_NNNX4_ix1 /5Phos/GACTNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/ 5Phos/3SpC3 GACT
MARS-Seq MARS_lig_NNNX4_ix2 /5Phos/CATGNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/ 5Phos/3SpC3 CATG
MARS-Seq MARS_lig_NNNX4_ix3 /5Phos/CCAANNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/ 5Phos/3SpC3 CCAA
MARS-Seq MARS_lig_NNNX4_ix4 /5Phos/CTGTNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/ 5Phos/3SpC3 CTGT
MARS-Seq MARS_lig_NNNX4_ix5 /5Phos/GTAGNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/ 5Phos/3SpC3 GTAG
MARS-Seq MARS_lig_NNNX4_ix6 /5Phos/TGATNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/ 5Phos/3SpC3 TGAT
Smart-seq2 Smart2-T30VN AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN N/A
Smart-seq2 Smart2_ISPCR AAGCAGTGGTATCAACGCAGAGT N/A
Smart-Seq2 Smart2-TSO AAGCAGTGGTATCAACGCAGAGTACATrGrG+G rGrG+G (LNA)
UMI-STRT-Seq C1-P1-RNA-TSO Bio-AAUGAUACGGCGACCACCGAUNNNNNGGG RNA
UMI-STRT-Seq C1-P1-T31 Bio-AATGATACGGCGACCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
UMI-STRT-Seq C1-P1-PCR-2 Bio-GAATGATACGGCGACCACCGAT
UMI-STRT-Seq C1-TN5-U PHO-CTGTCTCTTATACACATCTGACGC
Quartz-Seq RT primer (WTA) TATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTTTTTTTTTTTTTTTTTTTTTT N/A
Quartz-Seq Tagging primer TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTT N/A
Quartz-Seq Suppression primer (NH2)-GTATAGAATTCGCGGCCGCTCGCGAT

NexteraXT Primers[edit]

Primer Sequence Index Sequence
NexteraXT.N7id01 CAAGCAGAAGACGGCATACGAGATCGTGATGTCTCGTGGGCTCGG CGTGAT
NexteraXT.N7id02 CAAGCAGAAGACGGCATACGAGATACATCGGTCTCGTGGGCTCGG ACATCG
NexteraXT.N7id03 CAAGCAGAAGACGGCATACGAGATCGACTCGTCTCGTGGGCTCGG CGACTC
NexteraXT.N7id04 CAAGCAGAAGACGGCATACGAGATTGGTCAGTCTCGTGGGCTCGG TGGTCA
NexteraXT.N7id05 CAAGCAGAAGACGGCATACGAGATCACTGTGTCTCGTGGGCTCGG CACTGT
NexteraXT.N7id06 CAAGCAGAAGACGGCATACGAGATATTGGCGTCTCGTGGGCTCGG ATTGGC
NexteraXT.N7id07 CAAGCAGAAGACGGCATACGAGATGATCTGGTCTCGTGGGCTCGG GATCTG
NexteraXT.N7id08 CAAGCAGAAGACGGCATACGAGATTCAAGTGTCTCGTGGGCTCGG TCAAGT
NexteraXT.N7id09 CAAGCAGAAGACGGCATACGAGATGAGCTCGTCTCGTGGGCTCGG GAGCTC
NexteraXT.N7id10 CAAGCAGAAGACGGCATACGAGATAAGCTAGTCTCGTGGGCTCGG AAGCTA
NexteraXT.N7id11 CAAGCAGAAGACGGCATACGAGATGTAGCCGTCTCGTGGGCTCGG GTAGCC
NexteraXT.N7id12 CAAGCAGAAGACGGCATACGAGATTACAAGGTCTCGTGGGCTCGG TACAAG
NexteraXT.N7id13 CAAGCAGAAGACGGCATACGAGATTCATGGGTCTCGTGGGCTCGG TCATGG
NexteraXT.N7id14 CAAGCAGAAGACGGCATACGAGATTGTCTTGTCTCGTGGGCTCGG TGTCTT
NexteraXT.N7id15 CAAGCAGAAGACGGCATACGAGATAGGAAGGTCTCGTGGGCTCGG AGGAAG
NexteraXT.N7id16 CAAGCAGAAGACGGCATACGAGATAACCCCGTCTCGTGGGCTCGG AACCCC
NexteraXT.N7id17 CAAGCAGAAGACGGCATACGAGATGATGAGGTCTCGTGGGCTCGG GATGAG
NexteraXT.N7id18 CAAGCAGAAGACGGCATACGAGATTGAACTGTCTCGTGGGCTCGG TGAACT
NexteraXT.N7id19 CAAGCAGAAGACGGCATACGAGATTGCGTCGTCTCGTGGGCTCGG TGCGTC
NexteraXT.N7id20 CAAGCAGAAGACGGCATACGAGATGACAGGGTCTCGTGGGCTCGG GACAGG
NexteraXT.N7id21 CAAGCAGAAGACGGCATACGAGATGGGTTGGTCTCGTGGGCTCGG GGGTTG
NexteraXT.N7id22 CAAGCAGAAGACGGCATACGAGATTCCGAGGTCTCGTGGGCTCGG TCCGAG
NexteraXT.N7id23 CAAGCAGAAGACGGCATACGAGATTTTCGAGTCTCGTGGGCTCGG TTTCGA
NexteraXT.N7id24 CAAGCAGAAGACGGCATACGAGATGCGAATGTCTCGTGGGCTCGG GCGAAT
NexteraXT.S501 AATGATACGGCGACCACCGAGATCTACACTAGATCGCTCGTCGGCAGCGTC TAGATCGC


Sequencing Primers[edit]

Primer full name Primer full sequence Protocol Read
totoRNAseq Read 1 v2 CCACCGAGATCTACACTCTTTCCCTACACGACG totoRNAseq Read1
N2RevSeq2 CTCGGCATTCCTGCTGAACCGCTCTTCCGATCT totoRNAseq Read2
N2IndSeq AAGAGCGGTTCAGCAGGAATGCCGAG totoRNAseq N2 index Read
STRT-SEQ v3 GATCTAAGCAGTGGTATCAACGCAGAGTACGGG totoRNAseq Read1


Zhang Lab Indexes[edit]

Index List