Brandon:Protocols/egel protocol
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e gel protocol for size selection[edit]
- uses 2% agarose for the gel (needs 5-10 ng sample), get gel from gel container
1. place 2% agarose gel onto the machine
2. goto MODE --> preheat for 2 minutes
3. add in ladder and samples
ladder add 2x as much as TBE so you can see 3 uL loading dye 1 uL 1X TBE 2 uL ladder
samples (need 5-10 ng sample since agarose gel)
X uL sample X uL EB buffer _______ total 20 uL of sample
4. Check if middle wells for band extraction have liquid in them. If not, add 10-20 uL EB buffer
5. Run program 8, size select 2%. runs for 12 minutes. Run for longer as needed until band reaches pipetable well.
5. When band reaches well, pipet out into a .2 mL tube. Pipet up and down vigorously a few times to ensure most DNA is captured.
6. Now ready to send off for sequencing or the next application.