Brandon:Protocols/egel protocol

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e gel protocol for size selection[edit]

  • uses 2% agarose for the gel (needs 5-10 ng sample), get gel from gel container

1. place 2% agarose gel onto the machine


2. goto MODE --> preheat for 2 minutes


3. add in ladder and samples

ladder add 2x as much as TBE so you can see
3 uL loading dye
1 uL 1X TBE
2 uL ladder

samples (need 5-10 ng sample since agarose gel)

X uL sample
X uL EB buffer
_______
total 20 uL of sample


4. Check if middle wells for band extraction have liquid in them. If not, add 10-20 uL EB buffer


5. Run program 8, size select 2%. runs for 12 minutes. Run for longer as needed until band reaches pipetable well.


5. When band reaches well, pipet out into a .2 mL tube. Pipet up and down vigorously a few times to ensure most DNA is captured.


6. Now ready to send off for sequencing or the next application.