Brandon:Protocols/primers prep
Jump to navigation
Jump to search
Primers preparation[edit]
- for each nmol (nm) of primer add 10 uL nuclease free H2O. ie 35.5 nm, add 355 uL nuclease free water. now is at stock concentration of 100 uM (molar).
- next dilute to usable [] of 10 uM with 10X dilution.
- combine F/R primers in one tube for usable primer.