Brandon:Protocols/primers prep

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Primers preparation[edit]

  • for each nmol (nm) of primer add 10 uL nuclease free H2O. ie 35.5 nm, add 355 uL nuclease free water. now is at stock concentration of 100 uM (molar).
  • next dilute to usable [] of 10 uM with 10X dilution.
  • combine F/R primers in one tube for usable primer.