Daniel:Notebook/ComboLock/2016-11-21

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Sequencing Results-Miseq 2016-11-17[edit]

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Circularization Optimization Testing (Started Oct 31)[edit]

This was a repeat of the sequencing from 11-10, for which the results are analyzed on Nov 18) The base qualities and results were not great, so I tried to repeat it. However, we can see:

that the base qualities are all poor. Further, of the few sequences that actually aligned to the template, none of them are sufficiently out of the AmpF region (approximately bp 56). To make this calculation you can take the alignment position in column 4 and compare it against the number of matches in the CIGAR string (column 6) and see that 59 is about as high as it gets, which isn't necessarily far enough into the next region to determine its identity.

Cell Test with RCA (Started Nov 7)[edit]

Unfortunately, this winds up to the same problems as above. See the gallery below.

Once again, base qualities are generally poor and the matching region for the aligned bp does not leave the AmpF region. So this isn't helpful.