Daniel:Notebook/ComboLock/2016-12-6
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Sequencing Analysis[edit]
Base Qualities[edit]
After a few sequencing runs we finally have a run with good base quality, but the result is the same (11-21-16,11-18-16).
- Miseq20161205-BaseQual-ind30.png
Base qualities-index 30 (Circularization Optimization Testing)
- Miseq20161205-BaseQual-ind31.png
Base qualities-index 31 (Circularization Optimization Testing)
- Miseq20161205-BaseQual-ind25.png
Base qualities-index 25 (Cell RCA Testing)
So the base qualities are all pretty good.
SAM Analysis[edit]
Really not much analysis here. I'm just going to post the pictures since they tell the same story. The sequencing only really confirms the presence of AmpF and shows the strange product.
Aligned reads for index 30 (Phusion overnight ligation with RCA)
Aligned reads for index 31 (Phusion overnight ligation with RCA)