Daniel:Notebook/ComboLock/2016-3-20

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Room Temperature Polymerization Test[edit]

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The purpose of this test is to determine whether we can run most of the Drop Seq setup at room temperature. I will keep the aqueous phase on ice since normally this will have cells that we want to preserve. However, I will run the oil and device itself at room temperature. The outlet flow will also not run across the hotplate, thereby shortening the overall distance.

For this experiment I used the VA-004/gel loading dye mix from 03-15. This allows better visualization of the aqueous phase and has been shown to gel over time.

Run Parameters[edit]

  • Line 1: Oil (on ice); X psi; ~2 mL
  • Line 2: Aqueous (VA-044P; on ice); X psi; ~1 mL

Results[edit]