Daniel:Notebook/ComboLock/2016-5-28
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VIM Antibody Test[edit]
Protocol[edit]
- Ab-Oligo Mixing
- For VIM-PA and VIM-PB:
- Mix SMCC-treated antibodies (22 uL) with DTT-treated oligonucleotides (200 uL)
- Dilute probes to 75 ug/mL in PBS buffer with 4 mM EDTA, 35 ug/mL ssDNA, 0.1% fish gelatin, and 20 mM Tris HCl
Reagent | Stock | Final | Dilution | Volume in 1 mL (uL) |
VIM-PA | 530 ug/mL | 75 ug/mL | 7.1 | 141 |
EDTA | 500 mM | 4 mM | 125 | 8 |
Fish Gelatin | 100% | 0.10% | 1000 | 1 |
ssDNA (Calf Thymus) | 1 mg/mL | 35 ug/mL | 28.6 | 35 |
Tris HCl | 500 mM | 20 mM | 25 | 40 |
nf H2O | ------- | --------- | --------- | 810 |
Reagent | Stock | Final | Dilution | Volume in 1 mL (uL) |
VIM-PB | 600 ug/mL | 75 ug/mL | 8.0 | 125 |
EDTA | 500 mM | 4 mM | 125 | 8 |
Fish Gelatin | 100% | 0.10% | 1000 | 1 |
ssDNA (Calf Thymus) | 1 mg/mL | 35 ug/mL | 28.6 | 35 |
Tris HCl | 500 mM | 20 mM | 25 | 40 |
nf H2O | ------- | --------- | --------- | 826 |