Daniel:Notebook/ComboLock/2016-5-28

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VIM Antibody Test[edit]

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Protocol[edit]

  1. Ab-Oligo Mixing
    1. For VIM-PA and VIM-PB:
      1. Mix SMCC-treated antibodies (22 uL) with DTT-treated oligonucleotides (200 uL)
        1. Dilute probes to 75 ug/mL in PBS buffer with 4 mM EDTA, 35 ug/mL ssDNA, 0.1% fish gelatin, and 20 mM Tris HCl
        2. Reagent Stock Final Dilution Volume in 1 mL (uL)
          VIM-PA 530 ug/mL 75 ug/mL 7.1 141
          EDTA 500 mM 4 mM 125 8
          Fish Gelatin 100% 0.10% 1000 1
          ssDNA (Calf Thymus) 1 mg/mL 35 ug/mL 28.6 35
          Tris HCl 500 mM 20 mM 25 40
          nf H2O ------- --------- --------- 810
          Reagent Stock Final Dilution Volume in 1 mL (uL)
          VIM-PB 600 ug/mL 75 ug/mL 8.0 125
          EDTA 500 mM 4 mM 125 8
          Fish Gelatin 100% 0.10% 1000 1
          ssDNA (Calf Thymus) 1 mg/mL 35 ug/mL 28.6 35
          Tris HCl 500 mM 20 mM 25 40
          nf H2O ------- --------- --------- 826