Daniel:Notebook/ComboLock/2016-6-25
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Protocol[edit]
- Ethanol Precipitation
- Centrifuge at 4 C for 25 minutes at 14000 rpm
- Remove supernatant and add 750 uL chilled 75% EtOH
- Centrifuge for 15 minutes at minutes at 4 C at 14000 rpm
- Remove supernatant and dry the pellet in the hood
- Resuspend pellet in 20 uL volume nfH20
- Measure in Nanodrop
- Store DNA at 4C
Nanodrop Results[edit]
Batch Source
|
ng/uL
|
Batch2
|
43.1
|
Batch3
|
11.1
|
Batch5
|
36.1
|
Batch6
|
18.3
|