Daniel:Notebook/ComboLock/2016-7-13
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Million Fold Test (Started 7-11-16)[edit]
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Protocol[edit]
- Ethanol precipitation (from yesterday)
- Centrifuge at 4 C for 25 minutes at 14000 rpm
- Remove supernatant and add 750 uL chilled 75% EtOH
- Centrifuge for 15 minutes at minutes at 4 C at 14000 rpm
- Remove supernatant and dry the pellet in the hood
- Resuspend pellet in 20 uL volume nfH20
- Measure in Nanodrop
- Store DNA at 4C
Nanodrop Results[edit]
Source
|
ng/uL
|
Million Fold-Tube A
|
37.1
|
Million Fold-Tube B
|
30.1
|