Daniel:Notebook/ComboLock/2016-7-13

From ZhangLabWiki
Jump to navigation Jump to search

Million Fold Test (Started 7-11-16)[edit]

Back to Calendar

Protocol[edit]

  1. Ethanol precipitation (from yesterday)
    1. Centrifuge at 4 C for 25 minutes at 14000 rpm
    2. Remove supernatant and add 750 uL chilled 75% EtOH
    3. Centrifuge for 15 minutes at minutes at 4 C at 14000 rpm
    4. Remove supernatant and dry the pellet in the hood
    5. Resuspend pellet in 20 uL volume nfH20
    6. Measure in Nanodrop
    7. Store DNA at 4C

Nanodrop Results[edit]

Source ng/uL
Million Fold-Tube A 37.1
Million Fold-Tube B 30.1