Daniel:Notebook/ComboLock/2016-8-24
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Positive Control Amplicon Production (Started yesterday)[edit]
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Protocol[edit]
- Ethanol Precipitation
- Remove samples from -80C and centrifuge at 12000rpm at 4C for 25 minutes
- Remove the supernatant and add 750 uL chilled 70% EtOH
- Centrifuge at 12000rpm at 4C for 15 minutes
- Remove supernatant and add 50 uL nfH2O
- Combine A and B samples (100 uL total)
- Vacuum centrifuge; resuspend in 20 uL nfH2O
- Measure concentration in nanodrop
Nanodrop Results[edit]
Sample
|
ng/uL ssDNA
|
C1-C2 Amplicon
|
84.4
|
C3-C2 Amplicon
|
87.3
|