Daniel:Notebook/ComboLock/2016-8-24

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Positive Control Amplicon Production (Started yesterday)[edit]

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Protocol[edit]

  1. Ethanol Precipitation
    1. Remove samples from -80C and centrifuge at 12000rpm at 4C for 25 minutes
    2. Remove the supernatant and add 750 uL chilled 70% EtOH
    3. Centrifuge at 12000rpm at 4C for 15 minutes
    4. Remove supernatant and add 50 uL nfH2O
    5. Combine A and B samples (100 uL total)
    6. Vacuum centrifuge; resuspend in 20 uL nfH2O
    7. Measure concentration in nanodrop

Nanodrop Results[edit]

Sample ng/uL ssDNA
C1-C2 Amplicon 84.4
C3-C2 Amplicon 87.3