Daniel:Notebook/ComboLock/2016-9-6
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Protocol[edit]
- Ethanol Precipitation
- Pellet by centrifuging for 25 minutes at 12000rpm at 4C
- Remove supernatant and add 750 uL chilled 70% EtOH
- Centrifuge for 12 minutes at 12000rpm at 4C
- Remove supernatant and spin in vacuum centrifuge for 10 minutes
- Add 20 uL nfH2O and measure in nanodrop
Nanodrop Results[edit]
Sample
|
ng/uL
|
Sample A
|
31
|
Sample B
|
19.3
|
Sequencing Library[edit]
- Combined 15 uL from each of the two tubes above
- Stored at 4C in upcoming libraries box
- Sample DEJ-CL-PC03
Sample
|
ng/uL
|
uL added
|
final mass (ng)
|
9-6 Sample A
|
31
|
15
|
465
|
9-6 Sample B
|
19.3
|
15
|
289.5
|