Daniel:Notebook/ComboLock/2017-4-18

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Circularization Test (Started Jan 23, 2017)[edit]

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Sequencing Results[edit]

These just show another poorly hybridized product. The aligned bam files can be found in genome miner at:

/media/NAS3_volume2/Dan/combolock/miseq_20170414

Most of the reads have ~50bp matching, which is almost always the AmpF signal. I also discovered something upon close examination, shown in the read below. There is a small overlap at the final bases of LinkerR and first few bases of primer24RC. Apparently at high enough concentrations this causes trouble.

M00159:97:000000000-B4K7F:1:1101:11875:18466 ACTGTATTGTATTTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACCACTCTCAGATGTTATCGAGGTCCGACGATGTCAGACCGGGCCCACGGG ACTGTATTGTA TTTTTTTTTTTT AATGATACGGCGACCACCGA GATCTACACCACTCTCAGAT GTTATCGAGGTCCGACGATGTCAGAC CGGGCCCACGGG