Daniel:Notebook/GenomeMiner/2013-9-16
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Mock HL155 (Started 9/9/2013)[edit]
1% Substitutions[edit]
Found the error in the MATLAB script that gave the strange results from earlier. The script resused the same sequences for including error, meaning that the first few sequences had the right amount of error, but the error in each sequence compounded after each iteration. So by the end the sequences were basically garbage. The code has since been fixed.
Workflow[edit]
1. MockHL155_Master.m, Switch 2 2. scp v4s1_mockseq_1s.fq djacobse@132.239.135.41:/media/LTS_15T/DEJ_LTS/SeqStore/130628_HL155/mockseq/error1s/ 3. hl155bash.sh 4. perl imp_count_mismatch.plx (Matt's error counting script)
MATLAB Substitution Counting Results[edit]
1. MockHL155_Master.m, Switch 4
Perfect Reads: 1790246 (60.5 pct) Substitution Rate: 1479253 (1.0 pct) Low Matches: 450 (0.0 pct)
Alignment Results[edit]
2959000 reads; of these: 2959000 (100.00%) were unpaired; of these: 64665 (2.19%) aligned 0 times 2888951 (97.63%) aligned exactly 1 time 5384 (0.18%) aligned >1 times 97.81% overall alignment rate
So this is much more promising than the results from last week with errors.
Error Counting Results[edit]
Error Rate: 0.885% Error Rate of Insertions: 4.30e-05% Error Rate of Deletions: 2.08e-06% Error Rate of Substitutions: 0.885%
Mimic Data[edit]
Once again, this is based on the real data, with the numbers based on our previous calculations of error. Actual percentages: 1.01% substitutions, 0.26% insertions, 0.05% deletions
Workflow[edit]
1. MockHL155_Master.m, Switch 2 2. scp v4s1_mockseq_error_mimic.fq djacobse@132.239.135.41:/media/LTS_15T/DEJ_LTS/SeqStore/130628_HL155/mockseq/mimicerror/ 3. hl155bash.sh 4. perl imp_count_mismatch.plx (Matt's error counting script)
Alignment Results[edit]
2959000 reads; of these: 2959000 (100.00%) were unpaired; of these: 133942 (4.53%) aligned 0 times 2819919 (95.30%) aligned exactly 1 time 5139 (0.17%) aligned >1 times 95.47% overall alignment rate
Error Count Results[edit]
Error Rate: 1.08% Error Rate of Insertions: 0.195% Error Rate of Deletions: 5.52e-04% Error Rate of Substitutions: 0.887%