Daniel:Notebook/GenomeMiner/2013-9-23
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Mock HL155 (Started 9/9/2013)[edit]
Full Reference Sequence Reads[edit]
From Friday, I'm still trying to generate reads that use the entire reference sequence to generate the mock reads (important when deletions are present). Also, the current code does not allow 2 errors per base (but allows up to n errors per read where n is the number of bases in the read).
Workflow[edit]
1. MockHL155_Master.m, Switch 7 2. scp v4s1mockseq_extdel_errormimic.fq djacobse@132.239.135.41:/media/LTS_15T/DEJ_LTS/SeqStore/130628_HL155/mockseq/extdeletions/ 3. hl155bash.sh 4. perl imp_count_mismatch.plx
MATLAB Error Counts[edit]
1494539 substitutions (1.01 pct reads) 385509 insertions (0.26 pct reads) 74012 deletions (0.05 pct reads)
Alignment Results[edit]
2959000 reads; of these: 2959000 (100.00%) were unpaired; of these: 105936 (3.58%) aligned 0 times 2842493 (96.06%) aligned exactly 1 time 10571 (0.36%) aligned >1 times 96.42% overall alignment rate
Error Counting Results[edit]
Error Rate: 1.128% Error Rate of Insertions: 0.189% Error Rate of Deletions: 0.029% Error Rate of Substitutions: 0.91%