Daniel:Notebook/HiResChrPaint/2013-10-2
Jump to navigation
Jump to search
FISH (Started 10/01/2013)[edit]
Hybridization[edit]
Protocol[edit]
- Modified from original FISH protocol
Day 2
10. The next day, remove coverslip carefully and wash slides with 1 mL pre-warmed 2X SSCT at 60C for 15 minutes 11. Incubate slides in 1 mL 2X SSCT at room temperature for 10 minutes 12. Incubate slides in 1 mL 0.2X SSC at room temperature for 10 minutes 13. Tap slides dry with kimwipes; do not let the surface containing the cells touch the kimwipes 14. Add 15 uL mounting media such as SlowFadeGold with DAPI to center of dish 15. Cover the center of the dish with 22x22 #1.5 cover slip 16. Seal each slide using nail polish 17. Allow at least 30 minutes for nail polish to dry 18. Slides are quite stable for several months at 4C