Daniel:Notebook/HiResChrPaint/2013-10-2

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FISH (Started 10/01/2013)[edit]

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Hybridization[edit]

Protocol[edit]

Day 2

10. The next day, remove coverslip carefully and wash slides with 1 mL pre-warmed 2X SSCT at 60C for 15 minutes
11. Incubate slides in 1 mL 2X SSCT at room temperature for 10 minutes
12. Incubate slides in 1 mL 0.2X SSC at room temperature for 10 minutes
13. Tap slides dry with kimwipes; do not let the surface containing the cells touch the kimwipes
14. Add 15 uL mounting media such as SlowFadeGold with DAPI to center of dish
15. Cover the center of the dish with 22x22 #1.5 cover slip
16. Seal each slide using nail polish
17. Allow at least 30 minutes for nail polish to dry
18. Slides are quite stable for several months at 4C