Daniel:Notebook/HiResChrPaint/2013-12-15
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FISH Parameters[edit]
Hybridization[edit]
Day 2
Step | Dish 3 | Dish 4 |
10. | The next day, remove coverslip carefully and wash slides in pre-warmed 2X SSCT at 37C for 15 minutes | The next day, remove coverslip carefully and wash slides in pre-warmed 2X SSCT at 60C for 15 minutes |
11. | Incubate slides in 2X SSCT at room temperature for 10 minutes | Incubate slides in 2X SSCT at room temperature for 10 minutes |
12. | Incubate slides in 2X SSC at room temperature for 10 minutes | Incubate slides in 0.2X SSC at room temperature for 10 minutes |
13. | Wipe dry with kimwipes; do not let the surface containing the cells touch the kimwipes | Wipe dry with kimwipes; do not let the surface containing the cells touch the kimwipes |
14. | Add 15 uL DRAQ5 2uM to center | Add 15 uL DRAQ5 2uM to center |
15. | Remove media and allow to cool to room temperature | Remove media and allow to cool to room temperature |
16. | Cover with 22x22 #1.5 coverslip and seal using nail polish | Cover with 22x22 #1.5 coverslip and seal using nail polish |
17. | Allow at least 30 minutes for nail polish to dry | Allow at least 30 minutes for nail polish to dry |
18. | Slides are quite stable for several months at 4C | Slides are quite stable for several months at 4C |