Daniel:Notebook/HiResChrPaint/2013-12-15

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FISH Parameters[edit]

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Hybridization[edit]

Day 2

Step Dish 3 Dish 4
10. The next day, remove coverslip carefully and wash slides in pre-warmed 2X SSCT at 37C for 15 minutes The next day, remove coverslip carefully and wash slides in pre-warmed 2X SSCT at 60C for 15 minutes
11. Incubate slides in 2X SSCT at room temperature for 10 minutes Incubate slides in 2X SSCT at room temperature for 10 minutes
12. Incubate slides in 2X SSC at room temperature for 10 minutes Incubate slides in 0.2X SSC at room temperature for 10 minutes
13. Wipe dry with kimwipes; do not let the surface containing the cells touch the kimwipes Wipe dry with kimwipes; do not let the surface containing the cells touch the kimwipes
14. Add 15 uL DRAQ5 2uM to center Add 15 uL DRAQ5 2uM to center
15. Remove media and allow to cool to room temperature Remove media and allow to cool to room temperature
16. Cover with 22x22 #1.5 coverslip and seal using nail polish Cover with 22x22 #1.5 coverslip and seal using nail polish
17. Allow at least 30 minutes for nail polish to dry Allow at least 30 minutes for nail polish to dry
18. Slides are quite stable for several months at 4C Slides are quite stable for several months at 4C