Daniel:Notebook/HiResChrPaint/2013-3-27
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Probe Set Prep (Started 3/26/2013)[edit]
Ethanol Precipitation (From 3/26/2013)[edit]
4. Centrifuge at 3000 rpm for 30 min, 4 C 5. Remove Supernatant and add 600 uL chilled 75% EtOH 6. Transfer DNA pellet to a 1.5 mL tube 7. Spin at 14000 rpm for 5 min 8. Dry the pellet in the hood and resuspend with 100 uL ddH2O 9. Run 2 Qiaquick columns per sample, elute with 40 uL for each column 10. Pool samples (16 columns -> 8 samples) 11. Measure concentration with nanodrop
Probe | ng/uL | uL | ng |
V4S1 | 36.4 | 80 | 2912 |
V4S2 | 139.7 | 80 | 11176 |
V4S3 | 76.2 | 80 | 6096 |
V4S4 | 47.8 | 80 | 3824 |
V6S1 | 84.4 | 80 | 6752 |
V6S2 | 111.9 | 80 | 8952 |
V6S3 | 131.4 | 80 | 10512 |
V6S4 | 106.9 | 80 | 8552 |