Daniel:Notebook/HiResChrPaint/2013-4-1

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Probe Prep (Started 4/26/13)[edit]

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DpnII Digestion[edit]

Purpose: Trying to erase strange black marks from previous gels
1. Set up reactions according to table
Probe ng/uL uL 10x Buffer DpnII H2O Total
V4S1 41.6 30 5 5 10 50
V4S2 159.8 30 5 5 10 50
V4S3 73.1 30 5 5 10 50
V4S4 47.8 30 5 5 10 50
V6S1 84.4 30 5 5 10 50
V6S2 111.9 30 5 5 10 50
V6S3 131.4 30 5 5 10 50
V6S4 106.9 30 5 5 10 50
2. Incubate at 37 C for 1 hr; 20 minute heat shock at 65C
3. Column purification, elute with 30 uL
4. Measure concentration in Nanodrop

Nanodrop Results[edit]

Probe ng/uL uL ng
V4S1 21.9 30 657
V4S2 120.6 30 3618
V4S3 53.6 30 1608
V4S4 25 30 750
V6S1 61.2 30 1836
V6S2 82 30 2460
V6S3 105.3 30 3159
V6S4 80.6 30 2418

Gel Image[edit]

File:DpnDigest 040113.png