Daniel:Notebook/HiResChrPaint/2013-5-12
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Probe Prep Test 2 (Started 5/10/2013)[edit]
DpnII Digestion[edit]
3. Took from thermocycler 4. Column Purification, elute with 30 uL 5. Nanodrop
Nanodrop Results[edit]
Sample | V4S3A | V4S3B | V4S3C | V4S3D | V6S3A | V6S3B | V6S3C | V6S3D |
ng/uL | 115.1 | 138.1 | 120.3 | 124.4 | 138.9 | 131.4 | 133.5 | 127.3 |
ng in 30 uL | 3453 | 4143 | 3609 | 3732 | 4167 | 3942 | 4005 | 3819 |
Yield (%) | 67.7 | 66.3 | 69.3 | 71.4 | 73.0 | 71.0 | 68.4 | 71.6 |
Total Left (ug) | 14.9 | 15.9 |
Tomorrow will begin with a TBE gel to check band size. Following that will be lambda exonuclease digestion and ssColumn purification. Hopefully by the end of tomorrow I will have plenty of probe ready for dye coupling.