Daniel:Notebook/HiResChrPaint/2013-5-12

From ZhangLabWiki
Jump to navigation Jump to search

Probe Prep Test 2 (Started 5/10/2013)[edit]

Back to Calendar

DpnII Digestion[edit]

3. Took from thermocycler
4. Column Purification, elute with 30 uL
5. Nanodrop

Nanodrop Results[edit]

Sample V4S3A V4S3B V4S3C V4S3D V6S3A V6S3B V6S3C V6S3D
ng/uL 115.1 138.1 120.3 124.4 138.9 131.4 133.5 127.3
ng in 30 uL 3453 4143 3609 3732 4167 3942 4005 3819
Yield (%) 67.7 66.3 69.3 71.4 73.0 71.0 68.4 71.6
Total Left (ug) 14.9 15.9
Tomorrow will begin with a TBE gel to check band size.  Following that will be lambda exonuclease digestion and ssColumn purification.  
Hopefully by the end of tomorrow I will have plenty of probe ready for dye coupling.