Daniel:Notebook/HiResChrPaint/2013-5-15
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Probe Prep Test 2 (Started 5/10/2013)[edit]
TBU gel Quantification[edit]
1. Sample Matrix
Sample | V4S3 | V6S3 | Ladder | Ladder |
uL sample | 0.5 | 0.5 | 1 | 2 |
uL TBE | 19.5 | 19.5 | 1 | 0 |
uL 2X buffer | 20 | 20 | 2 | 2 |
2. Loading Matrix
Lane | 3 | 4 | 5 | 6 | 7 | 9 | 10 | 11 |
Sample | 2 uL Ladder | V4S3 | V6S3 | V4S3 | V6S3 | 1 uL Ladder | Matt | Matt |
Loaded | 4 | 2 | 2 | 4 | 4 | 4 | 4 | 4 |
Dilution | 1 | 1:40 | 1:40 | 1:20 | 1:20 | 1 | NA | NA |
3. Ran gel at 250V for 25 minutes 4. Stained with 3 uL SYBR gold for 20 minutes
Gel Results[edit]
Volume Report:File:GelQuant 051513.xls
File:GelQuant 051513 Volume.jpg
Quantification Results[edit]
File:GelQuant 051513 datafit.png
Sample | ng/uL | ug left (in 5 uL) |
V4S3 (1:40) | 1013.3 | 5.1 |
V6S3 (1:40) | 785.7 | 3.9 |
V4S3 (1:20) | 1571.1 | 7.9 |
V6S3(1:20) | 1448.9 | 7.2 |
Consensus
ng/uL | ug left (in 5 uL) | |
V4S3 | 1798.9 | 9.0 |
V6S3 | 1510.1 | 7.6 |
Discussion[edit]
Great amount of sample left! I am busy this week but will begin with a dye coupling Monday, and may even have images by the end of the week.