Daniel:Notebook/HiResChrPaint/2013-5-15

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Probe Prep Test 2 (Started 5/10/2013)[edit]

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TBU gel Quantification[edit]

1. Sample Matrix
Sample V4S3 V6S3 Ladder Ladder
uL sample 0.5 0.5 1 2
uL TBE 19.5 19.5 1 0
uL 2X buffer 20 20 2 2
2. Loading Matrix

Protocol 2

Lane 3 4 5 6 7 9 10 11
Sample 2 uL Ladder V4S3 V6S3 V4S3 V6S3 1 uL Ladder Matt Matt
Loaded 4 2 2 4 4 4 4 4
Dilution 1 1:40 1:40 1:20 1:20 1 NA NA
3. Ran gel at 250V for 25 minutes
4. Stained with 3 uL SYBR gold for 20 minutes

Gel Results[edit]

Volume Report:File:GelQuant 051513.xls

File:GelQuant 051513.jpg

File:GelQuant 051513 Volume.jpg

Quantification Results[edit]

File:GelQuant 051513 datafit.png

Sample ng/uL ug left (in 5 uL)
V4S3 (1:40) 1013.3 5.1
V6S3 (1:40) 785.7 3.9
V4S3 (1:20) 1571.1 7.9
V6S3(1:20) 1448.9 7.2

Consensus

  ng/uL ug left (in 5 uL)
V4S3 1798.9 9.0
V6S3 1510.1 7.6

Discussion[edit]

Great amount of sample left!  I am busy this week but will begin with a dye coupling Monday, and may even have images by the end of the week.