Daniel:Notebook/HiResChrPaint/2013-5-2

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Probe Prep Test (Started 4/22/2013)[edit]

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Dpn II Digest (from 5/1/2013)[edit]

3. Cut off incubation at 15:40 due to time constraints (still did 20 min heat shock)
4. Samples were crushed a little in the thermocycler, probably not a big deal but I might have lost some sample because I
  couldn't pipette it all out
5. Column purification; elute with 30 uL
6. Measured in Nanodrop

Results[edit]

Sample ng/uL ng in 30 uL Yield (%)
V4S2 39.6 1188 85.0
V6S2 30.6 918 82.0

Discussion[edit]

Great yield.  Will hopefully run the lambda exonuclease test this afternoon (followed by EtOH precipitation).  Might have to run it tomorrow.