Daniel:Notebook/HiResChrPaint/2013-5-2
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Probe Prep Test (Started 4/22/2013)[edit]
Dpn II Digest (from 5/1/2013)[edit]
3. Cut off incubation at 15:40 due to time constraints (still did 20 min heat shock) 4. Samples were crushed a little in the thermocycler, probably not a big deal but I might have lost some sample because I couldn't pipette it all out 5. Column purification; elute with 30 uL 6. Measured in Nanodrop
Results[edit]
Sample | ng/uL | ng in 30 uL | Yield (%) |
V4S2 | 39.6 | 1188 | 85.0 |
V6S2 | 30.6 | 918 | 82.0 |
Discussion[edit]
Great yield. Will hopefully run the lambda exonuclease test this afternoon (followed by EtOH precipitation). Might have to run it tomorrow.