Daniel:Notebook/HiResChrPaint/2013-6-1
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Probe Prep Test 3 (Started 5/31/2013)[edit]
EtOH Precipitation[edit]
1. Removed samples from -80C 2. Centrifuged for 30 min at 120000 rpm at 4C 3. Removed supernatant added 750 uL chilled EtOH 4. Centrifuged for 12 min at 120000 rpm at 4C 5. Removed supernatant, resuspended in 50 uL ddH20 6. Column Purification, eluted with 40 uL 7. Measured in Nanodrop, stored at 4C
Nanodrop Results[edit]
Normal Set w/aadUTP, alpha set[edit]
Sample | ng/uL | ug in 40 uL | total ug |
V4A | 130.3 | 5.2 | |
V4B | 134.6 | 5.4 | |
V4C | 130.8 | 5.2 | |
V4D | 130.4 | 5.2 | 21.0 |
V6A | 150 | 6.0 | |
V6B | 133.5 | 5.3 | |
V6C | 138.6 | 5.5 | |
V6D | 155.2 | 6.2 | 23.1 |
No aa-dUTP, Beta set[edit]
Sample | ng/uL | ug in 40 uL | total ug |
V4A | 147 | 5.9 | |
V4B | 125.7 | 5.0 | |
V4C | 116.1 | 4.6 | |
V4D | 124.9 | 5.0 | 20.5 |
V6A | 146.9 | 5.9 | |
V6B | 131.5 | 5.3 | |
V6C | 141.3 | 5.7 | |
V6D | 143 | 5.7 | 22.5 |