Daniel:Notebook/HiResChrPaint/2013-6-1

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Probe Prep Test 3 (Started 5/31/2013)[edit]

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EtOH Precipitation[edit]

1. Removed samples from -80C
2. Centrifuged for 30 min at 120000 rpm at 4C
3. Removed supernatant added 750 uL chilled EtOH
4. Centrifuged for 12 min at 120000 rpm at 4C
5. Removed supernatant, resuspended in 50 uL ddH20
6. Column Purification, eluted with 40 uL
7. Measured in Nanodrop, stored at 4C

Nanodrop Results[edit]

Normal Set w/aadUTP, alpha set[edit]

Sample ng/uL ug in 40 uL total ug
V4A 130.3 5.2
V4B 134.6 5.4
V4C 130.8 5.2
V4D 130.4 5.2 21.0
V6A 150 6.0
V6B 133.5 5.3
V6C 138.6 5.5
V6D 155.2 6.2 23.1

No aa-dUTP, Beta set[edit]

Sample ng/uL ug in 40 uL total ug
V4A 147 5.9
V4B 125.7 5.0
V4C 116.1 4.6
V4D 124.9 5.0 20.5
V6A 146.9 5.9
V6B 131.5 5.3
V6C 141.3 5.7
V6D 143 5.7 22.5