Daniel:Notebook/HiResChrPaint/2013-6-2
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Probe Prep 3 (Started 5/29/2013)[edit]
DpnII Digestion Test[edit]
Only running 1/4 of DNA I have for each sample to compare DpnII reaction yield using aadUTP DNA and normal DNA.
1. Set up
Sample | uL sample | uL Dpn II (50 U/uL) | uL 10x buffer | uL H20 | total |
alpha 4-A | 35 | 2 | 5 | 8 | 50 |
alpha 6-A | 35 | 2 | 5 | 8 | 50 |
beta 4-A | 35 | 2 | 5 | 8 | 50 |
beta 6-A | 35 | 2 | 5 | 8 | 50 |
2. Incubate in thermocycler at 37C for 2 hours; heat shock 20 minutes at 65 C 3. Column Purification, eluted with 30 uL EB 4. Stored at 4C; Gel running tomorrow
Nanodrop Results (from 6/4/2013)[edit]
Sample | ng/uL | ug in 30 uL | Yield (%) |
alpha-4A | 121.5 | 3.6 | 79.9 |
alpha-6A | 136.7 | 4.1 | 78.1 |
beta-4A | 126.4 | 3.8 | 73.7 |
beta-6A | 129.7 | 3.9 | 75.7 |