Daniel:Notebook/HiResChrPaint/2013-6-2

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Probe Prep 3 (Started 5/29/2013)[edit]

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DpnII Digestion Test[edit]

Only running 1/4 of DNA I have for each sample to compare DpnII reaction yield using aadUTP DNA and normal DNA.
1. Set up 
Sample uL sample uL Dpn II (50 U/uL) uL 10x buffer uL H20 total
alpha 4-A 35 2 5 8 50
alpha 6-A 35 2 5 8 50
beta 4-A 35 2 5 8 50
beta 6-A 35 2 5 8 50
2. Incubate in thermocycler at 37C for 2 hours; heat shock 20 minutes at 65 C
3. Column Purification, eluted with 30 uL EB
4. Stored at 4C; Gel running tomorrow

Nanodrop Results (from 6/4/2013)[edit]

Sample ng/uL ug in 30 uL Yield (%)
alpha-4A 121.5 3.6 79.9
alpha-6A 136.7 4.1 78.1
beta-4A 126.4 3.8 73.7
beta-6A 129.7 3.9 75.7