Daniel:Notebook/HiResChrPaint/2013-8-13

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FISH (Started 8/12/2013)[edit]

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Hybridization (From 8/12/2013)[edit]

Day 2

10. The next day, remove coverslip carefully and wash slides in pre-warmed jar containing 2X SSCT at 60C for 15 minutes
11. Incubate slides in 2X SSCT at room temperature for 10 minutes
12. Incubate slides in 0.2X SSC at room temperature for 10 minutes
13. Tap slides dry on papertowels; do not let the surface containing the cells touch the paper towels
14. Add 15 uL mounting media such as SlowFadeGold with DAPI to a 22x30 #1.5 coverslip
15. Invert slides onto coverslips with mounting media
16. Seal each slide using nail polish
17. Allow at least 30 minutes for nail polish to dry
18. Slides are quite stable for several months at 4C

Microscopy[edit]

We did microscopy on the Olympus upstairs to screen before we used the confocal. It didn't look great.