Daniel:Notebook/HiResChrPaint/2013-8-13
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FISH (Started 8/12/2013)[edit]
Hybridization (From 8/12/2013)[edit]
Day 2
10. The next day, remove coverslip carefully and wash slides in pre-warmed jar containing 2X SSCT at 60C for 15 minutes 11. Incubate slides in 2X SSCT at room temperature for 10 minutes 12. Incubate slides in 0.2X SSC at room temperature for 10 minutes 13. Tap slides dry on papertowels; do not let the surface containing the cells touch the paper towels 14. Add 15 uL mounting media such as SlowFadeGold with DAPI to a 22x30 #1.5 coverslip 15. Invert slides onto coverslips with mounting media 16. Seal each slide using nail polish 17. Allow at least 30 minutes for nail polish to dry 18. Slides are quite stable for several months at 4C
Microscopy[edit]
We did microscopy on the Olympus upstairs to screen before we used the confocal. It didn't look great.