Daniel:Notebook/HiResChrPaint/2013-8-9
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S2 Probe Prep (ULS; Started 8/8/2013)[edit]
DpnII Digestion (Started 8/8/2013)[edit]
3. Column purification, elute with 30 uL EB 4. Measure in Nanodrop
Nanodrop Results[edit]
Sample | V4S2AB | V4S2CD | V6S2A | V6S2B | V6S2C | V6S2D |
ng/uL | 109.3 | 122.9 | 115.4 | 117 | 121 | 111.1 |
ug in 30 uL | 3.3 | 3.7 | 3.5 | 3.5 | 3.6 | 3.3 |
Total ug | 7.0 | 13.9 |
Gel Image
Lambda Exonuclease Digestion[edit]
1. Reaction Table
Sample | V4S1C | V4S1D | V6S1A | V6S1B | V6S1C | V6S1D |
ng/uL | 109.3 | 122.9 | 115.4 | 117 | 121 | 111.1 |
ug in 25 uL | 2.7 | 3.1 | 2.9 | 2.9 | 3.0 | 2.8 |
uL added | 25 | 25 | 25 | 25 | 25 | 25 |
uL 10x buffer | 5 | 5 | 5 | 5 | 5 | 5 |
uL Lambda Exonuclease (5U/uL) | 3 | 3 | 3 | 3 | 3 | 3 |
uL nfH2O | 17 | 17 | 17 | 17 | 17 | 17 |
total | 50 | 50 | 50 | 50 | 50 | 50 |
2. Incubate for 1 hour at 37C followed by 10 minute heat inactivation at 75C 3. Pool samples 4. ssDNA column purification, elute with 20 uL 5. Measure Concentration in Nanodrop
Nanodrop Results[edit]
ng/uL | ug in 20 uL | |
V4S2 | 94.7 | 1.9 |
V6S2 | 195.2 | 3.9 |
FISH[edit]
Alan and I prepped some slides for FISH. However, we accidentally used the wrong media for attaching to slides. The cells did not attach well even after about 4 hours. So Alan and I decided to try again Monday.