Daniel:Notebook/HiResChrPaint/2013-9-11

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Fibroblast Adherence Timecourse (Started 09/09/2013)[edit]

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Petri dishes are in! So I've got a revised itinerary. First, a recap. There are a few important variables to track in this experiment:

  • (1) surface type (slide or dish)
  • (2) trypsin dilution (to ensure cells may attach)
  • (3) Cell seeding density (1,000,000 cells/mL recommended)
  • (4) Adding more media to help growth

New Template for Experiment

Name Surface Trypsin Dilution Cell Seeding Density Media Kicker
Slide 09/09/13 Slide 1:10 1.00E+06 None
Slide 1 Slide 1:20 1.00E+06 None
Slide 2 Slide 1:20 1.00E+06 100 uL DMEM
Dish 1 Dish 1:20 1.00E+06 None
Dish 2 Dish 1:20 1.00E+06 100 uL DMEM

Timecourse Experiment 09/11/2013[edit]

1. Cultured cells from 9/9/13 (step 6) (PGP1F, Passage 12)
2. Aspirate media and rinse with 5 mL PBS
3. Aspirate PBS and add 1 mL trypsin solution; incubate for several minutes
4. Add 9 mL DMEM to cell culture, mix by gently pipetting
5. Add 100 uL cell suspension to center of surface (2x slides, 2x dishes)
6. Took time course photos of slides at 3 hours and 6 hours
7. Continued tomorrow