Daniel:Notebook/HiResChrPaint/2013-9-11
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Fibroblast Adherence Timecourse (Started 09/09/2013)[edit]
Petri dishes are in! So I've got a revised itinerary. First, a recap. There are a few important variables to track in this experiment:
- (1) surface type (slide or dish)
- (2) trypsin dilution (to ensure cells may attach)
- (3) Cell seeding density (1,000,000 cells/mL recommended)
- (4) Adding more media to help growth
New Template for Experiment
Name | Surface | Trypsin Dilution | Cell Seeding Density | Media Kicker |
Slide 09/09/13 | Slide | 1:10 | 1.00E+06 | None |
Slide 1 | Slide | 1:20 | 1.00E+06 | None |
Slide 2 | Slide | 1:20 | 1.00E+06 | 100 uL DMEM |
Dish 1 | Dish | 1:20 | 1.00E+06 | None |
Dish 2 | Dish | 1:20 | 1.00E+06 | 100 uL DMEM |
Timecourse Experiment 09/11/2013[edit]
1. Cultured cells from 9/9/13 (step 6) (PGP1F, Passage 12) 2. Aspirate media and rinse with 5 mL PBS 3. Aspirate PBS and add 1 mL trypsin solution; incubate for several minutes 4. Add 9 mL DMEM to cell culture, mix by gently pipetting 5. Add 100 uL cell suspension to center of surface (2x slides, 2x dishes) 6. Took time course photos of slides at 3 hours and 6 hours 7. Continued tomorrow