Daniel:Notebook/HiResChrPaint/2014-1-25

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Alu Control (Started 01/08/2014)[edit]

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Alu Control FISH (Started 01/24/2014)[edit]

Day 2

10. The next day, remove coverslip carefully and wash slides in pre-warmed 2X SSCT at 60C for 15 minutes
11. Incubate in 2X SSCT at room temperature for 10 minutes
12. Incubate in 0.2X SSC at room temperature for 10 minutes
13. Wipe gently with kimwipe; do not let the surface containing the cells touch the kimwipe
14. Add 15 uL DRAQ5 to center of dish
15. Cover with 22x22 #1.5 coverslip
16. Seal each slide using nail polish
17. Allow at least 30 minutes for nail polish to dry
18. Stored at 4C (Microscopy Results)