Daniel:Notebook/HiResChrPaint/2014-7-18

From ZhangLabWiki
Jump to navigation Jump to search

Dystrophin Probes[edit]

Back to Calendar

The dystrophin probes I designed last week arrived from IDT. Attached are their specs:

The oligos arrived as dry oligos in a plate. The calculations I made are in the following text file:

Oligo Pool Synthesis[edit]

  1. Resuspend each oligo to make them all 1 mM (See Plate Specs for values)
  2. Combine 2 uL from each oligo
    1. Makes 12.5 ug/uL total pool
    2. Total volume will be 64 uL
  3. Add 742.4 uL nfH2O, dilutes to 1 ug/uL total pool
    1. Create dilution series and measure in nanodrop

Nanodrop Results[edit]

Dilution (X:1) ng/uL proj. ug/uL
5 79.6 0.40
10 38.2 0.38
20 19.3 0.39
50 7.4 0.37
100 3.7 0.37
Average   0.38

So I guess my calculations were a bit off, but 0.4 ug/uL is pretty good for FISH, especially considering I need 30 pmol per experiment (0.4 ug/uL ~60 pmol/uL).