Daniel:Notebook/HiResChrPaint/2014-8-17

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Padlock Probes (Started 8/17/2014)[edit]

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Lambda Exonuclease Digestion[edit]

Reaction Table

Reagent uL
Amplicon 70
Lambda Exonuclease Buffer (10X) 10
Lambda Exonuclease 5
nf H2O 15
Total 100
  1. Set up reaction according to table
  2. Incubate at 37C for 1 hour
  3. Purify using 2 ssDNA columns, elute with 20 uL nfH2O

Results[edit]

Sample ng/uL ug in 20 uL ug Total
PPRL-XO-A 10.5 0.2
PPRL-XO-B 20.8 0.4 0.6

So there is very little left. I'm going to do a bigger amplification tomorrow, and I'll pool today's samples with tomorrow's before continuing with USER/DpnII.