Daniel:Notebook/HiResChrPaint/2014-9-22
Slide FISH (Started 09/19/2014)[edit]
Microscopy[edit]
Dystrophin[edit]
- 20140922 DMD488 Cot1546.lif Field1 ch00.jpg
Field 1
- 20140922 DMD488 Cot1546.lif Field2 ch00.jpg
Field 2
- 20140922 DMD488 Cot1546.lif Field3 ch00.jpg
Field 3
- 20140922 DMD488 Cot1546.lif Zoom1 ch00.jpg
Zoom 1
These images don't tell me a great deal one way or another. There appears to be good dystrophin signal, so I think that the probes must be working, but it is difficult to check whether or not that signal is dystrophin itself. The Cot1 continues to perform admirably.
Chr21 Padlock Probes[edit]
- 20140922 PPRL488 Cot1546.lif Field1 NoThreshold ch00.jpg
No Threshold
- 20140922 PPRL488 Cot1546.lif Field1 NormalThreshold ch00.jpg
Normal Zero Threshold (20/255)
- 20140922 PPRL488 Cot1546.lif Field1 BestThreshold ch00.jpg
Best Threshold (100/255)
These images have much stronger green signal, corresponding to the Padlock Probes from Rui (PPRL). The comforting thing is that since there are thousands of these probes, I would expect a stronger signal. However, they are only supposed to paint chromosome 21, and even then not the whole thing. Therefore I should only see 2 or so concentrated dots, but I typically see much stronger signal than that. Tomorrow's microscopy may provide more insight.