Daniel:Notebook/RNAFACS/2015-10-30
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RNA Scope Trial Run 5: 10X-I[edit]
Microcscopy Results[edit]
So I tried to look at the results under the 10X. Unfortunately, I checked and DAPI quenches DRAQ5. There is a lot of signal under the DAPI channel, but I cannot visualize the nuclei, which makes it very difficult to take images.
- 20151030.lif Series001Snapshot1 ch00.jpg
Field 1
- 20151030.lif Series003Snapshot1 ch00.jpg
Field 2
- 20151030.lif Series004Snapshot1 ch00.jpg
Field 3; several nuclei in this frame, only 1 shows both FEZF2 and RBFOX3 signal