Daniel:Notebook/RNAFACS/2015-4-12
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In Tube FISH Test (Started 4/10/2015)[edit]
Washing[edit]
- Add 50 uL of wash buffer and mix thoroughly
- Pellet by centrifugation (1000g for 5 min, 4C)
- Resuspend cells in 50 uL wash buffer for 30 minutes at 30C
- Pellet by centrifugation and resuspend cells in 50 uL wash buffer for 30 minutes at 30C
- Pellet cells and resuspend in 15 uL Slow Fade Gold + DAPI, mount on slides; seal with nail polish