Daniel:Notebook/RNAFACS/2015-4-12

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In Tube FISH Test (Started 4/10/2015)[edit]

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Washing[edit]

  1. Add 50 uL of wash buffer and mix thoroughly
  2. Pellet by centrifugation (1000g for 5 min, 4C)
  3. Resuspend cells in 50 uL wash buffer for 30 minutes at 30C
  4. Pellet by centrifugation and resuspend cells in 50 uL wash buffer for 30 minutes at 30C
  5. Pellet cells and resuspend in 15 uL Slow Fade Gold + DAPI, mount on slides; seal with nail polish